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SS-B抗原の解析とELISAによる臨床応用
https://doi.org/10.15114/smj.59.357
https://doi.org/10.15114/smj.59.3571fe7fb19-c229-42eb-925d-39f63f1f168c
名前 / ファイル | ライセンス | アクション |
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||
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公開日 | 2019-08-16 | |||||||
タイトル | ||||||||
言語 | ja | |||||||
タイトル | SS-B抗原の解析とELISAによる臨床応用 | |||||||
タイトル | ||||||||
言語 | en | |||||||
タイトル | Analysis of SS-B Antigen and the Clinical Application using Enzyme-Linked Immunosorbent Assay | |||||||
言語 | ||||||||
言語 | jpn | |||||||
キーワード | ||||||||
言語 | en | |||||||
主題Scheme | Other | |||||||
主題 | SS-B antigen | |||||||
キーワード | ||||||||
言語 | en | |||||||
主題Scheme | Other | |||||||
主題 | Rabbit thymus extract (RTE). | |||||||
キーワード | ||||||||
言語 | en | |||||||
主題Scheme | Other | |||||||
主題 | Sjogren's syndrome (SjS) | |||||||
キーワード | ||||||||
言語 | en | |||||||
主題Scheme | Other | |||||||
主題 | Enzyme-linked immunosorbent assay (ELISA). | |||||||
キーワード | ||||||||
言語 | en | |||||||
主題Scheme | Other | |||||||
主題 | Western blotting | |||||||
資源タイプ | ||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
資源タイプ | departmental bulletin paper | |||||||
ID登録 | ||||||||
ID登録 | 10.15114/smj.59.357 | |||||||
ID登録タイプ | JaLC | |||||||
著者 |
細川, 寿和
× 細川, 寿和
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著者別名 | ||||||||
姓名 | Hosokawa, Toshikazu | |||||||
抄録 | ||||||||
内容記述タイプ | Abstract | |||||||
内容記述 | SS-B antigen was purified from a saline extract of acetone powder of rabbit thymus (RTE) by a salting out method using ammonium sulfate, which was followed by affinity chromatography using Glycosyl-hardgel tagged with anti-SS-B antibody, and then the antigen fractions corresponding to anti-SS-B antibodies in patient sera were analized by Western blotting. An enzymelinked immunosorbent assay (ELISA) was developed using purified 40kDa SS-B antigen and the titers of anti-SS-B antibodies in sera were determined and compared with laboratory findings in patients with collagen diseases. The results obtained were as follows. 1) Affinity-purified SS-B antigen showed three bands, 50kDa, 40kDa and 28kDa, reactive with anti-SS-B antibody positive sera in Western blotting. The 40kDa fraction reacted only with anti-SS-B antibody, whereas the 50kDa fraction reacted with both the anti-SS-B antibody and anti-human IgG (Fc) antibody. 2) It is suggested that the 50kDa fraction has at least two antigenic epitopes: one is common to that of the 40kDa fraction reacting with anti-SS-B antibody and the other is similar to the Fc portion of human IgG. 3) The 40kDa SS-B fraction was obtained from gel slices by electrophoretical elution after SDS-polyacrylamide gel electrophoresis. Enzyme-linked immunosorbent assay using this fraction enabled us to detect IgG-, IgA-, and IgM-type anti-40kDa SS-B antibodies in sera of patients with collagen diseases. Although antibodies of all Ig classes were detected, the highest value was obtained for IgG-type. IgG-type anti-40kDa SS-B antibodies were found in 24(70.5%) of 34 patients with SjS, 13(44.8%) of 29 patients with SLE and 2(20%) of 10 patients with PSS-PM. The incidence and titer of the antibody were higher in patients of SjS than in patients other collagen diseases. In SjS and SLE, IgG-type anti-SS-B positive patients were associated with the appearance of leukopenia, lymphopenia, and hyper γ-globulinemia (p <0.05). | |||||||
書誌情報 |
札幌医学雑誌 = The Sapporo medical journal en : The Sapporo medical journal 巻 59, 号 4, p. 357-368, 発行日 1990-08-01 |
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ISSN | ||||||||
収録物識別子タイプ | ISSN | |||||||
収録物識別子 | 0036-472X | |||||||
著者版フラグ | ||||||||
出版タイプ | VoR | |||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
出版者 | ||||||||
出版者 | 札幌医科大学医学部 |