@article{oai:sapmed.repo.nii.ac.jp:00014867,
 author = {細川, 寿和},
 issue = {4},
 journal = {札幌医学雑誌 = The Sapporo medical journal, The Sapporo medical journal},
 month = {Aug},
 note = {SS-B antigen was purified from a saline extract of acetone powder of rabbit thymus （RTE） by a salting out method using ammonium sulfate, which was followed by affinity chromatography using Glycosyl-hardgel tagged with anti-SS-B antibody, and then the antigen fractions corresponding to anti-SS-B antibodies in patient sera were analized by Western blotting. An enzymelinked immunosorbent assay （ELISA） was developed using purified 40kDa SS-B antigen and the titers of anti-SS-B antibodies in sera were determined and compared with laboratory findings in patients with collagen diseases. The results obtained were as follows. 1） Affinity-purified SS-B antigen showed three bands, 50kDa, 40kDa and 28kDa, reactive with anti-SS-B antibody positive sera in Western blotting. The 40kDa fraction reacted only with anti-SS-B antibody, whereas the 50kDa fraction reacted with both the anti-SS-B antibody and anti-human IgG （Fc） antibody. 2） It is suggested that the 50kDa fraction has at least two antigenic epitopes: one is common to that of the 40kDa fraction reacting with anti-SS-B antibody and the other is similar to the Fc portion of human IgG. 3） The 40kDa SS-B fraction was obtained from gel slices by electrophoretical elution after SDS-polyacrylamide gel electrophoresis. Enzyme-linked immunosorbent assay using this fraction enabled us to detect IgG-, IgA-, and IgM-type anti-40kDa SS-B antibodies in sera of patients with collagen diseases. Although antibodies of all Ig classes were detected, the highest value was obtained for IgG-type. IgG-type anti-40kDa SS-B antibodies were found in 24（70.5%） of 34 patients with SjS, 13（44.8%） of 29 patients with SLE and 2（20%） of 10 patients with PSS-PM. The incidence and titer of the antibody were higher in patients of SjS than in patients other collagen diseases. In SjS and SLE, IgG-type anti-SS-B positive patients were associated with the appearance of leukopenia, lymphopenia, and hyper γ-globulinemia （p <0.05）.},
 pages = {357--368},
 title = {SS-B抗原の解析とELISAによる臨床応用},
 volume = {59},
 year = {1990}
}