{"created":"2023-05-15T09:07:28.960567+00:00","id":14867,"links":{},"metadata":{"_buckets":{"deposit":"17d35b2a-9d74-410a-8b3c-4afe908cd337"},"_deposit":{"created_by":14,"id":"14867","owners":[14],"pid":{"revision_id":0,"type":"depid","value":"14867"},"status":"published"},"_oai":{"id":"oai:sapmed.repo.nii.ac.jp:00014867","sets":["1658:1659:1721"]},"author_link":["26529","26528"],"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1990-08-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"368","bibliographicPageStart":"357","bibliographicVolumeNumber":"59","bibliographic_titles":[{"bibliographic_title":"札幌医学雑誌 = The Sapporo medical journal"},{"bibliographic_title":"The Sapporo medical journal","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"SS-B antigen was purified from a saline extract of acetone powder of rabbit thymus （RTE） by a salting out method using ammonium sulfate, which was followed by affinity chromatography using Glycosyl-hardgel tagged with anti-SS-B antibody, and then the antigen fractions corresponding to anti-SS-B antibodies in patient sera were analized by Western blotting. An enzymelinked immunosorbent assay （ELISA） was developed using purified 40kDa SS-B antigen and the titers of anti-SS-B antibodies in sera were determined and compared with laboratory findings in patients with collagen diseases. The results obtained were as follows. 1） Affinity-purified SS-B antigen showed three bands, 50kDa, 40kDa and 28kDa, reactive with anti-SS-B antibody positive sera in Western blotting. The 40kDa fraction reacted only with anti-SS-B antibody, whereas the 50kDa fraction reacted with both the anti-SS-B antibody and anti-human IgG （Fc） antibody. 2） It is suggested that the 50kDa fraction has at least two antigenic epitopes: one is common to that of the 40kDa fraction reacting with anti-SS-B antibody and the other is similar to the Fc portion of human IgG. 3） The 40kDa SS-B fraction was obtained from gel slices by electrophoretical elution after SDS-polyacrylamide gel electrophoresis. Enzyme-linked immunosorbent assay using this fraction enabled us to detect IgG-, IgA-, and IgM-type anti-40kDa SS-B antibodies in sera of patients with collagen diseases. Although antibodies of all Ig classes were detected, the highest value was obtained for IgG-type. IgG-type anti-40kDa SS-B antibodies were found in 24（70.5%） of 34 patients with SjS, 13（44.8%） of 29 patients with SLE and 2（20%） of 10 patients with PSS-PM. The incidence and titer of the antibody were higher in patients of SjS than in patients other collagen diseases. In SjS and SLE, IgG-type anti-SS-B positive patients were associated with the appearance of leukopenia, lymphopenia, and hyper γ-globulinemia （p <0.05）.","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"26529","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Hosokawa, Toshikazu"}]}]},"item_7_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15114/smj.59.357","subitem_identifier_reg_type":"JaLC"}]},"item_7_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"札幌医科大学医学部"}]},"item_7_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0036-472X","subitem_source_identifier_type":"ISSN"}]},"item_7_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"細川, 寿和"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-16"}],"displaytype":"detail","filename":"n0036472X594357.pdf","filesize":[{"value":"2.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"n0036472X594357.pdf","url":"https://sapmed.repo.nii.ac.jp/record/14867/files/n0036472X594357.pdf"},"version_id":"f5c3d01d-adfc-4c93-bf34-a98371f2c2d1"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"SS-B antigen","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Rabbit thymus extract (RTE).","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Sjogren's syndrome (SjS)","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Enzyme-linked immunosorbent assay (ELISA).","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Western blotting","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"SS-B抗原の解析とELISAによる臨床応用","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"SS-B抗原の解析とELISAによる臨床応用","subitem_title_language":"ja"},{"subitem_title":"Analysis of SS-B Antigen and the Clinical Application using Enzyme-Linked Immunosorbent Assay","subitem_title_language":"en"}]},"item_type_id":"7","owner":"14","path":["1721"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2019-08-16"},"publish_date":"2019-08-16","publish_status":"0","recid":"14867","relation_version_is_last":true,"title":["SS-B抗原の解析とELISAによる臨床応用"],"weko_creator_id":"14","weko_shared_id":-1},"updated":"2023-12-13T02:24:29.120393+00:00"}