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Cloning of a cDNA for the Human Cell Adhesion Kinase β
https://doi.org/10.15114/tr.30.37
https://doi.org/10.15114/tr.30.374b59e4ce-c7f5-46d8-9423-55a01b42a6e1
名前 / ファイル | ライセンス | アクション |
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n004140933037.pdf (515.3 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2019-07-31 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Cloning of a cDNA for the Human Cell Adhesion Kinase β | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Cell adhesion kinase β (CAKβ) | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Focal adhesion kinase (FAK) | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Autophosphorylation site | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Src homology 2 and 3 (SH-2 and SH-3) domains | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
ID登録 | ||||||
ID登録 | 10.15114/tr.30.37 | |||||
ID登録タイプ | JaLC | |||||
著者 |
Sasaki, Hiroko
× Sasaki, Hiroko× Nagura, Kazuko× Ishino, Masaho× Ohba, Takeaki× Matsuya, Manabu× Sasaki, Terukatsu |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Cell adhesion kinase beta (CAKbeta) is the second protein-tyrosine kinase (PTK) of the focal adhesion kinase (FAK) subfamily with large N- and C-domains in addition to the central kinase domain but without Src homology 2 and 3 (SH-2 and SH-3) domains. In this paper, cloning and sequencing of a cDNA encoding human CAKβ are described. A full-length clone (clone B) contained 4,157- base pairs of human CAKβ cDNA including 243-base pairs of the 5'-untranslated sequence and 881-base pairs of the 3'-untranslated sequence with a polyadenyla-tion signal (ATTAAA). The clone B of human CAKβ cDNA has an open read-ing frame encoding 1009 amino acid residues ; the human CAKβ has the same number of amino acid residues in the N-, C-, and kinase-domains as rat CAKβ . The amino acid sequence of human CAKβ is 95.4% identical with that of rat CAKβ . The species difference is most prominent in the C-domain. All three previously-recognized, subfamily-specific residues in the kinase domains of FAK and the rat CAKβ are also found in the human CAKβ . The residues V??? and A???, which have been considered to be characteristic to CAKβ , are found to be conserved also in the human CAK? . It has been postulated that CAKβ is important as a docking protein. The autophosphorylation site and also the ligand site to the SH-2 domains of the Src-family PTKs, Y???AEI, are found to be conserved in the human CAKβ . The ligand sequence for the Grb2 SH-2 domain, Y???HNV of the rat CAKβ , is found functionally conserved in the human CAKβ , Y???LNV. The third ligand sequence, E???PPPKPSR, participating in the binding to the SH-3 domains of pp130cas and Efs, is also found conserved in the human CAKβ . The extreme N- terminal 88 amino acid residues of the rat CAKβ were previously found entirely different from FAK and found unique to CAK? . Ninety four percent of those 88 residues in the human CAKβ are found identical with the rat CAKβ . This high sequence homology strongly suggeststhat this region is involved in the specific function of CAKβ different from FAK. | |||||
書誌情報 |
Tumor Research en : Tumor Research 巻 30, p. 37-46, 発行日 1995 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0041-4093 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | Sapporo Medical University |