| Item type |
紀要論文 / Departmental Bulletin Paper(1) |
| 公開日 |
2019-07-31 |
| タイトル |
|
|
タイトル |
Expression of VEGF-C and VEGF-D mRNA Levels Using Real-Time Quantitative RT-PCR in Lymph Node Metastasis of Human Gastric Carcinomas |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
VEGF-C |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
VEGF-D |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Real-time PCR |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Lymph node metastasis |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Gastric carcinoma |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| ID登録 |
|
|
ID登録 |
10.15114/tr.37.33 |
|
ID登録タイプ |
JaLC |
| 著者 |
Furuhata, Tomohisa
Kawakami, Masayo
Kimura, Yasutoshi
Kihara, Chikashi
Okita, Kenji
Yamaguchi, Koji
Hata, Fumitake
Mukaiya, Mitsuhiro
Katsuramaki, Tadashi
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
This study was undertaken to determine whether expressions of the vascular endothelial growth factor C (VEGF-C) and D (VEGF-D) correlate with clinicopathological parameters, with particular reference to lymph node metastasis in gastric carcinoma. Based on the data, as a preliminary study, we investigated whether VEGFC expression could be a predictor of lymph node metastasis. Twenty-two surgical specimens of gastric carcinomas with (n=14) or without (n=8) lymph node metastasis were studied. The mRNA levels of VEGF-C and VEGF-D were quantified using real-time quantitative (RTQ) RT-PCR, a new method of kinetic quantitative PCR. We then calculated the tumor:normal VEGF-C and VEGF-D mRNA level ratios for each specimen which were designated as the normalized mRNA value. The normalized mRNA values of VEGF-C in primary tumors correlated well with lymph node metastasis but that of VEGF-D did not. We then arbitrarily chose the cut-off point for the normalized mRNA values of VEGF-C based on the mRNA values of patients without lymph node metastasis (mean + SD); we examined the data to see if the values above the cut-off point would indicate the presence of lymph node metastasis. The sensitivity and specificity of the normalized mRNA values of VEGF-C for the diagnosis of lymph node metastasis were 85.2% and 75.0%, respectively. These results suggest that the VEGF-C may play an important role in lymph node metastasis in gastric carcinoma and that VEGF-C may be a useful diagnostic marker for lymph node metastasis. |
| 書誌情報 |
Tumor Research
en : Tumor Research
巻 37,
p. 33-40,
発行日 2002
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0041-4093 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 出版者 |
|
|
出版者 |
Sapporo Medical University |
n0041409337133.pdf (92.4 kB)
