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Expression of VEGF-C and VEGF-D mRNA Levels Using Real-Time Quantitative RT-PCR in Lymph Node Metastasis of Human Gastric Carcinomas
https://doi.org/10.15114/tr.37.33
https://doi.org/10.15114/tr.37.33c89fef66-1b8d-4dda-95f9-e009c4d1dd50
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
n0041409337133.pdf (92.4 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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| 公開日 | 2019-07-31 | |||||
| タイトル | ||||||
| タイトル | Expression of VEGF-C and VEGF-D mRNA Levels Using Real-Time Quantitative RT-PCR in Lymph Node Metastasis of Human Gastric Carcinomas | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | VEGF-C | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | VEGF-D | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Real-time PCR | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Lymph node metastasis | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Gastric carcinoma | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| ID登録 | ||||||
| ID登録 | 10.15114/tr.37.33 | |||||
| ID登録タイプ | JaLC | |||||
| 著者 |
Furuhata, Tomohisa
× Furuhata, Tomohisa× Kawakami, Masayo× Kimura, Yasutoshi× Kihara, Chikashi× Okita, Kenji× Yamaguchi, Koji× Hata, Fumitake× Mukaiya, Mitsuhiro× Katsuramaki, Tadashi |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | This study was undertaken to determine whether expressions of the vascular endothelial growth factor C (VEGF-C) and D (VEGF-D) correlate with clinicopathological parameters, with particular reference to lymph node metastasis in gastric carcinoma. Based on the data, as a preliminary study, we investigated whether VEGFC expression could be a predictor of lymph node metastasis. Twenty-two surgical specimens of gastric carcinomas with (n=14) or without (n=8) lymph node metastasis were studied. The mRNA levels of VEGF-C and VEGF-D were quantified using real-time quantitative (RTQ) RT-PCR, a new method of kinetic quantitative PCR. We then calculated the tumor:normal VEGF-C and VEGF-D mRNA level ratios for each specimen which were designated as the normalized mRNA value. The normalized mRNA values of VEGF-C in primary tumors correlated well with lymph node metastasis but that of VEGF-D did not. We then arbitrarily chose the cut-off point for the normalized mRNA values of VEGF-C based on the mRNA values of patients without lymph node metastasis (mean + SD); we examined the data to see if the values above the cut-off point would indicate the presence of lymph node metastasis. The sensitivity and specificity of the normalized mRNA values of VEGF-C for the diagnosis of lymph node metastasis were 85.2% and 75.0%, respectively. These results suggest that the VEGF-C may play an important role in lymph node metastasis in gastric carcinoma and that VEGF-C may be a useful diagnostic marker for lymph node metastasis. | |||||
| 書誌情報 |
Tumor Research en : Tumor Research 巻 37, p. 33-40, 発行日 2002 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0041-4093 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 出版者 | ||||||
| 出版者 | Sapporo Medical University | |||||
