{"created":"2023-05-15T09:06:58.476294+00:00","id":14181,"links":{},"metadata":{"_buckets":{"deposit":"6f3e9d7c-7438-4f23-9993-c77962a7f521"},"_deposit":{"created_by":3,"id":"14181","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"14181"},"status":"published"},"_oai":{"id":"oai:sapmed.repo.nii.ac.jp:00014181","sets":["1658:1661:1664"]},"author_link":["23447","23450","23445","23452","23448","23446","23451","23449","23453"],"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2002","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"40","bibliographicPageStart":"33","bibliographicVolumeNumber":"37","bibliographic_titles":[{"bibliographic_title":"Tumor Research"},{"bibliographic_title":"Tumor Research","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"This study was undertaken to determine whether expressions of the vascular endothelial growth factor C (VEGF-C) and D (VEGF-D) correlate with clinicopathological parameters, with particular reference to lymph node metastasis in gastric carcinoma. Based on the data, as a preliminary study, we investigated whether VEGFC expression could be a predictor of lymph node metastasis. Twenty-two surgical specimens of gastric carcinomas with (n=14) or without (n=8) lymph node metastasis were studied. The mRNA levels of VEGF-C and VEGF-D were quantified using real-time quantitative (RTQ) RT-PCR, a new method of kinetic quantitative PCR. We then calculated the tumor:normal VEGF-C and VEGF-D mRNA level ratios for each specimen which were designated as the normalized mRNA value. The normalized mRNA values of VEGF-C in primary tumors correlated well with lymph node metastasis but that of VEGF-D did not. We then arbitrarily chose the cut-off point for the normalized mRNA values of VEGF-C based on the mRNA values of patients without lymph node metastasis (mean + SD); we examined the data to see if the values above the cut-off point would indicate the presence of lymph node metastasis. The sensitivity and specificity of the normalized mRNA values of VEGF-C for the diagnosis of lymph node metastasis were 85.2% and 75.0%, respectively. These results suggest that the VEGF-C may play an important role in lymph node metastasis in gastric carcinoma and that VEGF-C may be a useful diagnostic marker for lymph node metastasis.","subitem_description_type":"Abstract"}]},"item_7_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15114/tr.37.33","subitem_identifier_reg_type":"JaLC"}]},"item_7_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Sapporo Medical University"}]},"item_7_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0041-4093","subitem_source_identifier_type":"ISSN"}]},"item_7_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Furuhata, Tomohisa"}],"nameIdentifiers":[{"nameIdentifier":"23445","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kawakami, Masayo"}],"nameIdentifiers":[{"nameIdentifier":"23446","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kimura, Yasutoshi"}],"nameIdentifiers":[{"nameIdentifier":"23447","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kihara, Chikashi"}],"nameIdentifiers":[{"nameIdentifier":"23448","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okita, Kenji"}],"nameIdentifiers":[{"nameIdentifier":"23449","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamaguchi, Koji"}],"nameIdentifiers":[{"nameIdentifier":"23450","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hata, Fumitake"}],"nameIdentifiers":[{"nameIdentifier":"23451","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mukaiya, Mitsuhiro"}],"nameIdentifiers":[{"nameIdentifier":"23452","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Katsuramaki, Tadashi"}],"nameIdentifiers":[{"nameIdentifier":"23453","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-07-31"}],"displaytype":"detail","filename":"n0041409337133.pdf","filesize":[{"value":"92.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"n0041409337133.pdf","url":"https://sapmed.repo.nii.ac.jp/record/14181/files/n0041409337133.pdf"},"version_id":"2f210c61-5e2d-4f34-823a-fc8a35146863"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"VEGF-C","subitem_subject_scheme":"Other"},{"subitem_subject":"VEGF-D","subitem_subject_scheme":"Other"},{"subitem_subject":"Real-time PCR","subitem_subject_scheme":"Other"},{"subitem_subject":"Lymph node metastasis","subitem_subject_scheme":"Other"},{"subitem_subject":"Gastric carcinoma","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Expression of VEGF-C and VEGF-D mRNA Levels Using Real-Time Quantitative RT-PCR in Lymph Node Metastasis of Human Gastric Carcinomas","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Expression of VEGF-C and VEGF-D mRNA Levels Using Real-Time Quantitative RT-PCR in Lymph Node Metastasis of Human Gastric Carcinomas"}]},"item_type_id":"7","owner":"3","path":["1664"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-07-31"},"publish_date":"2019-07-31","publish_status":"0","recid":"14181","relation_version_is_last":true,"title":["Expression of VEGF-C and VEGF-D mRNA Levels Using Real-Time Quantitative RT-PCR in Lymph Node Metastasis of Human Gastric Carcinomas"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-12-13T02:43:35.785322+00:00"}