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RT-PCR法によるヒトサイトメガロウイルス前初期遺伝子mRNAの検出
https://doi.org/10.15114/smj.62.193
https://doi.org/10.15114/smj.62.193ace0950a-19a7-4ee1-834d-7c33b9ad254d
名前 / ファイル | ライセンス | アクション |
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n0036472X624193.pdf (2.4 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2019-07-31 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Detection of Human Cytomegalovirus Immediate Early Gene-Specific mRNA by Reverse Transcription Polymerase Chain Reaction. | |||||
タイトル | ||||||
言語 | ja | |||||
タイトル | RT-PCR法によるヒトサイトメガロウイルス前初期遺伝子mRNAの検出 | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Human cytomegalovirus | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | RT-PCR | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Immediate early gene | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | mRNA | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
ID登録 | ||||||
ID登録 | 10.15114/smj.62.193 | |||||
ID登録タイプ | JaLC | |||||
著者 |
Nagata, Nobuo
× Nagata, Nobuo× Yoshida, Koichi× Shimada, Masamitsu× Numazaki, Kei× Chiba, Shunzo× Fujinaga, Kei |
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著者別名 | ||||||
姓名 | 長田, 伸夫 | |||||
著者別名 | ||||||
姓名 | 吉田, 幸一 | |||||
著者別名 | ||||||
姓名 | 罵田, 雅光 | |||||
著者別名 | ||||||
姓名 | 沼崎, 啓 | |||||
著者別名 | ||||||
姓名 | 千葉, 峻三 | |||||
著者別名 | ||||||
姓名 | 藤永, 意 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We have established a method for detection of human cytomegalovirus (HCMV) immediate early gene (IE)-specific mRNA by reverse transcription polymerase chain reaction (RT-PCR). Using the IE-1 specific primer fragments designed for RT-PCR, we have amplified a 232 base-pair fragment which represents IE-1 mRNA transcribed from the IE-1 gene of HCMV AD169 strain. No DNA cross reactivities to other human herpes virus DNAs or human embryonic lung cell (MRC-5 line) DNA were observed. The RT-PCR assay indicated HCMV IE-1-specific mRNA in HCMV-infected MRC-5 cells and in peripheral blood specimens from one of 3 patients examined. The results indicate that RT-PCR will be readily applicable to rapid detection of HCMV mRNA and may be useful for diagnosis of active primary or recurrent HCMV infections. | |||||
書誌情報 |
札幌医学雑誌 = The Sapporo medical journal en : The Sapporo medical journal 巻 62, 号 4, p. 193-201, 発行日 1993-08-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0036-472X | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | 札幌医科大学医学部 |