@article{oai:sapmed.repo.nii.ac.jp:00014792,
 author = {Nagata, Nobuo and Yoshida, Koichi and Shimada, Masamitsu and Numazaki, Kei and Chiba, Shunzo and Fujinaga, Kei},
 issue = {4},
 journal = {札幌医学雑誌 = The Sapporo medical journal, The Sapporo medical journal},
 month = {Aug},
 note = {We have established a method for detection of human cytomegalovirus （HCMV） immediate early gene （IE）-specific mRNA by reverse transcription polymerase chain reaction （RT-PCR）. Using the IE-1 specific primer fragments designed for RT-PCR, we have amplified a 232 base-pair fragment which represents IE-1 mRNA transcribed from the IE-1 gene of HCMV AD169 strain. No DNA cross reactivities to other human herpes virus DNAs or human embryonic lung cell （MRC-5 line） DNA were observed. The RT-PCR assay indicated HCMV IE-1-specific mRNA in HCMV-infected MRC-5 cells and in peripheral blood specimens from one of 3 patients examined. The results indicate that RT-PCR will be readily applicable to rapid detection of HCMV mRNA and may be useful for diagnosis of active primary or recurrent HCMV infections.},
 pages = {193--201},
 title = {Detection of Human Cytomegalovirus Immediate Early Gene-Specific mRNA by Reverse Transcription Polymerase Chain Reaction.},
 volume = {62},
 year = {1993}
}