{"created":"2023-05-15T09:07:25.781896+00:00","id":14792,"links":{},"metadata":{"_buckets":{"deposit":"d27f68ec-02f6-414c-90c9-e64554030bae"},"_deposit":{"created_by":14,"id":"14792","owners":[14],"pid":{"revision_id":0,"type":"depid","value":"14792"},"status":"published"},"_oai":{"id":"oai:sapmed.repo.nii.ac.jp:00014792","sets":["1658:1659:1724"]},"author_link":["26201","26202","26203","26204","26205","26206","26195","26196","26197","26198","26199","26200"],"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1993-08-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"201","bibliographicPageStart":"193","bibliographicVolumeNumber":"62","bibliographic_titles":[{"bibliographic_title":"札幌医学雑誌 = The Sapporo medical journal"},{"bibliographic_title":"The Sapporo medical journal","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We have established a method for detection of human cytomegalovirus （HCMV） immediate early gene （IE）-specific mRNA by reverse transcription polymerase chain reaction （RT-PCR）. Using the IE-1 specific primer fragments designed for RT-PCR, we have amplified a 232 base-pair fragment which represents IE-1 mRNA transcribed from the IE-1 gene of HCMV AD169 strain. No DNA cross reactivities to other human herpes virus DNAs or human embryonic lung cell （MRC-5 line） DNA were observed. The RT-PCR assay indicated HCMV IE-1-specific mRNA in HCMV-infected MRC-5 cells and in peripheral blood specimens from one of 3 patients examined. The results indicate that RT-PCR will be readily applicable to rapid detection of HCMV mRNA and may be useful for diagnosis of active primary or recurrent HCMV infections.","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"26201","nameIdentifierScheme":"WEKO"}],"names":[{"name":"長田, 伸夫"}]},{"nameIdentifiers":[{"nameIdentifier":"26202","nameIdentifierScheme":"WEKO"}],"names":[{"name":"吉田, 幸一"}]},{"nameIdentifiers":[{"nameIdentifier":"26203","nameIdentifierScheme":"WEKO"}],"names":[{"name":"罵田, 雅光"}]},{"nameIdentifiers":[{"nameIdentifier":"26204","nameIdentifierScheme":"WEKO"}],"names":[{"name":"沼崎, 啓"}]},{"nameIdentifiers":[{"nameIdentifier":"26205","nameIdentifierScheme":"WEKO"}],"names":[{"name":"千葉, 峻三"}]},{"nameIdentifiers":[{"nameIdentifier":"26206","nameIdentifierScheme":"WEKO"}],"names":[{"name":"藤永, 意"}]}]},"item_7_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15114/smj.62.193","subitem_identifier_reg_type":"JaLC"}]},"item_7_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"札幌医科大学医学部"}]},"item_7_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0036-472X","subitem_source_identifier_type":"ISSN"}]},"item_7_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nagata, Nobuo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yoshida, Koichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shimada, Masamitsu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Numazaki, Kei"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Chiba, Shunzo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fujinaga, Kei"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-07-31"}],"displaytype":"detail","filename":"n0036472X624193.pdf","filesize":[{"value":"2.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"n0036472X624193.pdf","url":"https://sapmed.repo.nii.ac.jp/record/14792/files/n0036472X624193.pdf"},"version_id":"d7486c1c-c0d2-4258-b163-4f45bfd2417c"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Human cytomegalovirus","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"RT-PCR","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Immediate early gene","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"mRNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Detection of Human Cytomegalovirus Immediate Early Gene-Specific mRNA by Reverse Transcription Polymerase Chain Reaction.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Detection of Human Cytomegalovirus Immediate Early Gene-Specific mRNA by Reverse Transcription Polymerase Chain Reaction.","subitem_title_language":"en"},{"subitem_title":"RT-PCR法によるヒトサイトメガロウイルス前初期遺伝子mRNAの検出","subitem_title_language":"ja"}]},"item_type_id":"7","owner":"14","path":["1724"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2019-07-31"},"publish_date":"2019-07-31","publish_status":"0","recid":"14792","relation_version_is_last":true,"title":["Detection of Human Cytomegalovirus Immediate Early Gene-Specific mRNA by Reverse Transcription Polymerase Chain Reaction."],"weko_creator_id":"14","weko_shared_id":-1},"updated":"2023-12-13T02:23:48.099848+00:00"}