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Dexmedetomidine attenuates surgery-induced cognitive deficit and hippocampal Mapt expression in aged mice
https://doi.org/10.15114/smj.88.65
https://doi.org/10.15114/smj.88.65246337f6-bde0-4d59-8d48-6c8a2e57a48c
名前 / ファイル | ライセンス | アクション |
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0036472X88165.pdf (1.5 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2020-04-07 | |||||
タイトル | ||||||
タイトル | Dexmedetomidine attenuates surgery-induced cognitive deficit and hippocampal Mapt expression in aged mice | |||||
言語 | en | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | postoperative cognitive deficit | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | hippocampus | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | mRNA expression | |||||
キーワード | ||||||
言語 | ja | |||||
主題Scheme | Other | |||||
主題 | transcriptome analysis | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | surgical stress | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | dexmedetomidine | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
ID登録 | ||||||
ID登録 | 10.15114/smj.88.65 | |||||
ID登録タイプ | JaLC | |||||
著者 |
Shunsuke, TACHIBANA
× Shunsuke, TACHIBANA× Tomo, HAYASE× Michiaki, YAMAKAGE |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Background Postoperative cognitive dysfunction is a significant complication, but the mechanisms underlying this condition and its impact on the brain network are not fully elucidated. The goals of this study were to verify how the transcriptional network in the hippocampus of aged mice changes following sevoflurane/surgery-induced stress and to substantiate how dexmedetomidine influences the cognitive function and mRNA changes in the hippocampus.Materials and methods We first performed transcriptome analysis to confirm the changes of mRNA expression in the Naïve and Ope (surgery under sevoflurane) groups. Then, the mice were divided into four groups: Naïve, Sevo (sevoflurane exposure), Ope, and Dex (dexmedetomidine injection before surgery). We selected the Mapt gene, the upregulated expression of which has been observed in our transcriptome analysis and in neurodegenerative disorders, as the target gene and investigated whether the changes in its expression occurred in the hippocampus using quantitative rev transcription polymerase chain reaction (qRT-PCR). The cognitive function of mice was evaluated via the Barnes Maze test.Results In the qRT-PCR analysis, Mapt expression was significantly upregulated (2.60 ± 0.77 in mice from the Ope group vs. 1.00 ± 0.11 in mice from the Naïve group [mean ± SD for fold change]; p<0.01). Dexmedetomidine significantly attenuated the sevoflurane/surgery-induced upregulation of Mapt expression in the hippocampus (0.88 ± 0.32; p<0.01). Sevoflurane/surgery-induced stress also increased the time to identify the target box, and dexmedetomidine treatment inhibited the time extension 7, 14, and 28 days after the surgery.Conclusions Dexmedetomidine attenuates the sevoflurane/surgery-induced cognitive deficit and Mapt expression in the hippocampus of aged mice. | |||||
書誌情報 |
札幌医学雑誌=The Sapporo Medical Journal en : The Sapporo Medical Journal 巻 88, 号 1-6, p. 65-76, 発行日 2019-12-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0036-472X | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | 札幌医科大学 |