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Methods for Fractionation and Identification of Nonhistone Proteins of Rat Liver Chromatin
https://doi.org/10.15114/smj.47.563
https://doi.org/10.15114/smj.47.563ed6cfee6-8fcf-47a7-8fbd-b6dcd7c9fb58
名前 / ファイル | ライセンス | アクション |
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n0036472X476563.pdf (2.7 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2019-08-23 | |||||
タイトル | ||||||
タイトル | Methods for Fractionation and Identification of Nonhistone Proteins of Rat Liver Chromatin | |||||
言語 | en | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
ID登録 | ||||||
ID登録 | 10.15114/smj.47.563 | |||||
ID登録タイプ | JaLC | |||||
著者 |
Kunihiko, Ishitani
× Kunihiko, Ishitani× Ichiro, Urushizaki× Irving, Listowsky |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Combinations of methods were devised for fractionation and characterization of proteins obtained from rat liver chromatin. The chromosomal proteins were extracted from DNA by treatment with 3M NaCl, 7M urea, or by heating in 1% sodium dodecyl sulfate. The proteins dissociatdd by sodium dodecyl sulfate were fractionated by gel filtration in the presence of the detergent. A number of discrete components, each containing a population of polypeptides of limited heterogeneity with respect to molecular weight, were isolated by this method. Proteins obtained by urea-salt dissociation were initially subfractionated into histone and nonhistone components. The nonhistone proteins were examined by sodium dodecyl polyacrylamide gel electrophoresis and by analytical and preparative isoelectric focusing methods, in an effort to provide reproducible methods for identification of specific families of proteins. | |||||
書誌情報 |
札幌医学雑誌 = The Sapporo medical journal en : The Sapporo medical journal 巻 47, 号 6, p. 563-570, 発行日 1978-12-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0036-472X | |||||
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出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | 札幌医科大学医学部 |