WEKO3
-
RootNode
アイテム
抗CD8モノクローナル抗体VH領域の解析
https://doi.org/10.15114/smj.62.31
https://doi.org/10.15114/smj.62.31b86c761d-e812-4109-a3a0-c64deef2c7e9
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
n0036472X62131.pdf (567.7 kB)
|
|
| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2019-07-31 | |||||||||
| タイトル | ||||||||||
| タイトル | Analysis of the Immunoglobulin Heavy Chain Variable Region of Hybridomas Producing anti-CD8 Monoclonal Antibodies. | |||||||||
| 言語 | en | |||||||||
| タイトル | ||||||||||
| タイトル | 抗CD8モノクローナル抗体VH領域の解析 | |||||||||
| 言語 | ja | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Somatic mutation | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Affinity maturation | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Complementarity determining region (CDR) | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Immunoglobulin variable region gene | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | departmental bulletin paper | |||||||||
| ID登録 | ||||||||||
| ID登録 | 10.15114/smj.62.31 | |||||||||
| ID登録タイプ | JaLC | |||||||||
| 著者 |
Sato, Toshiya
× Sato, Toshiya
× Kon, Shinichiro
|
|||||||||
| 著者別名 | ||||||||||
| 識別子Scheme | WEKO | |||||||||
| 識別子 | 26146 | |||||||||
| 姓名 | 佐藤, 俊哉 | |||||||||
| 著者別名 | ||||||||||
| 識別子Scheme | WEKO | |||||||||
| 識別子 | 26147 | |||||||||
| 姓名 | 今, 信一郎 | |||||||||
| 抄録 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | We analyzed the nucleotide sequence of immunoglobulin （Ig） heavy chain variable regions （VH） of seven hybridomas which produce anti-CD8 monoclonal antibodies. These seven antibodies were classified into four groups according to the results of a cell binding inhibition assay. The assay also indicated that CD8 antigen expressed at least four different epitopes. The VH nucleotide sequences were classified in three families. Three clones belonged to VH subgroup I（B）, and two to VH subgroup V（A）. Sequences in members of each family showed identical V-D-J recombinations, suggesting that the clones of each family arose from common ancestral clones. Some somatic mutations were observed in both the framework segments and the complementarity determining regions （CDR） of the I（B） clones, and only one amino acid substitution was observed. Two clones of the V（A） subgroup possessed an identical VH sequence. Although the clones of each family had originated from single ancestral clones, reactivity within families with CD8 antigen was found to vary widely. At the same time, two clones derived from different fusions, on the basis of their equal reactivities with CD8, were classified into subgroup II（A） ; subsequently, they were shown to share the same V-D-J combination. The speeds of reaction of the antibodies with CD8 antigen were investigated by flow cytometry. The observed rates of increase of brightness and final saturated brightness with these antibodies were quite different. Those results indicated that somatic mutations occurring in the VH gene of the anti-CD8 antibodies cause differences in their affinity with epitopes. Two separately originated clones that were induced by the same CD8 antigen showed identical paratopes and VH usage. | |||||||||
| 書誌情報 |
札幌医学雑誌 = The Sapporo medical journal en : The Sapporo medical journal 巻 62, 号 1, p. 31-41, 発行日 1993-02-01 |
|||||||||
| ISSN | ||||||||||
| 収録物識別子タイプ | ISSN | |||||||||
| 収録物識別子 | 0036-472X | |||||||||
| 著者版フラグ | ||||||||||
| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
| 出版者 | ||||||||||
| 出版者 | 札幌医科大学医学部 | |||||||||
