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新生仔ラット心筋細胞の分離・培養について
https://doi.org/10.15114/smj.61.155
https://doi.org/10.15114/smj.61.15549cc73fb-eacb-4489-a68d-265441901d0a
名前 / ファイル | ライセンス | アクション |
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n0036472X612155.pdf (3.0 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2019-07-31 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | A Simple Isolation Method and Primary Culture of Neonatal Rat Cardiac Myocytes | |||||
タイトル | ||||||
言語 | ja | |||||
タイトル | 新生仔ラット心筋細胞の分離・培養について | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Neonatal rat cardiac myocytes | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Cultured myocytes | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Seeding density | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Myofilaments | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Intermediate filaments | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
ID登録 | ||||||
ID登録 | 10.15114/smj.61.155 | |||||
ID登録タイプ | JaLC | |||||
著者 |
Kimura, Hisakazu
× Kimura, Hisakazu× Furukawa, Kazunori× Mochizuki, Yohichi× Ohshika, Hideyo |
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著者別名 | ||||||
姓名 | 木村, 永一 | |||||
著者別名 | ||||||
姓名 | 古川, 一典 | |||||
著者別名 | ||||||
姓名 | 望月, 洋一 | |||||
著者別名 | ||||||
姓名 | 大鹿, 英世 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | For long-term maintenance of functional cardiac myocytes in primary culture, we developed a simple method for isolation of cells from neonatal Wistar rat ventricles. The highest viable cell yield was obtained when using 13 to 20 ventricles for one isolation and employing col lagenase as the tissue-digesting enzyme at 200 U/ml. Examination of the seeding density revealed that, at a density of 1.5XlO? cells/35-mm dish, the cells were arranged into long, thick, fibrous masses exhibiting synchronous contraction with a constant rate for up to 20 days in culture, indicat ing that this density was appropriate for maintaining functional cardiac myocytes in culture. The cells at Day 7 of culture were stimulated by addition of 1X10??M isoproterenol, leading to an increase in the beating rate, but the beating rate of the cells at Day 14 decreased somewhat by the addition of isoproterenol. Electron microscopic examinations of the myocytes at Day 3 clarified the reconstitution of the myofibrils with the formation of Z-lines, accompanied by the formation of intercalated discs. The formation of these contractile structures was maintained throughout the 20 days in primary culture. The intermediate filaments, desmin and vimentin, were detected in the cultured myocytes by the immunofluorescence method, showing a cross-striated pattern. It is likely that both intermediate filaments were involved in the reconstitution and maintenance of the striated structures of the myofibrils. These results suggest that the neonatal rat cardiac myocytes isolated and cultured by the present method are useful for an in vitro experimental system of the heart. | |||||
書誌情報 |
札幌医学雑誌 = The Sapporo medical journal en : The Sapporo medical journal 巻 61, 号 2, p. 155-163, 発行日 1992-04-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0036-472X | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | 札幌医科大学医学部 |