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The present study was designed to determine the acting site, and secondarily, to evaluate the role of the kallikrein-kinin system on renal hemodynamics and prostaglandin E? (PGE?) production. IgG (anti-KAL-IgG) and Fab fragment (anti-KAL-Fab) were isolated from monoclonal antibody against rat glandular kallikrein ; both anti-KAL-IgG and anti-KAL-Fab were found to inhibit glandular kallikrein activity in vitro. Next, purified IgG (anti-ricin-IgG) and Fab fragment (anti-ricin-Fab) from monoclonal antibody to ricin, which is a vegetable protein and does not exist in mammals, were tested ; neither anti-ricin-IgG nor anti-ricin-Fab blocked kallkrein activity in vitro. The anti-KAL-Fab were observed to pass through the glomeruli and reach the luminal site of the distal nephron, but anti-KAL-IgG did not pass through the glomerulus. In this study, both anti-KAL-Fab and anti- KAL-IgG were administered to unanesthetized normotensive rats. 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非麻酔下正常血圧ラットにおける腎Kallikrein-Kinin系抑制時の腎水・ナトリウム代謝
https://doi.org/10.15114/smj.62.75
https://doi.org/10.15114/smj.62.7531bf6cc6-9c45-4970-a463-c6a5aaa37700
名前 / ファイル | ライセンス | アクション |
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n0036472X62275.pdf (751.0 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2019-07-31 | |||||
タイトル | ||||||
言語 | ja | |||||
タイトル | 非麻酔下正常血圧ラットにおける腎Kallikrein-Kinin系抑制時の腎水・ナトリウム代謝 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | The Effects of the Suppression of Renal Kallikrein-Kinin System on the Water and Sodium Metabolism in Normotensive Conscious Rats | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Kallikrein-kinin system | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Water-sodium metabolism | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Prostaglandin E? | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Monoclonal antibody | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Fab-fragment | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
ID登録 | ||||||
ID登録 | 10.15114/smj.62.75 | |||||
ID登録タイプ | JaLC | |||||
著者 |
斎藤, 重幸
× 斎藤, 重幸× 島本, 和明× Oscar, A. Carretero |
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著者別名 | ||||||
姓名 | Saitoh, Shigeyuki | |||||
著者別名 | ||||||
姓名 | Shimamoto, Kazuaki | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Recent findings have suggested that the endogenous renal kallikrein-kinin system acts on the renal water-sodium metabolism at the distal nephron as a paracrine hormone system. The present study was designed to determine the acting site, and secondarily, to evaluate the role of the kallikrein-kinin system on renal hemodynamics and prostaglandin E? (PGE?) production. IgG (anti-KAL-IgG) and Fab fragment (anti-KAL-Fab) were isolated from monoclonal antibody against rat glandular kallikrein ; both anti-KAL-IgG and anti-KAL-Fab were found to inhibit glandular kallikrein activity in vitro. Next, purified IgG (anti-ricin-IgG) and Fab fragment (anti-ricin-Fab) from monoclonal antibody to ricin, which is a vegetable protein and does not exist in mammals, were tested ; neither anti-ricin-IgG nor anti-ricin-Fab blocked kallkrein activity in vitro. The anti-KAL-Fab were observed to pass through the glomeruli and reach the luminal site of the distal nephron, but anti-KAL-IgG did not pass through the glomerulus. In this study, both anti-KAL-Fab and anti- KAL-IgG were administered to unanesthetized normotensive rats. When 1.0 mg anti-KAL-Fab was administered, urinary kallikrein activity (kininogenase activity) and the excretion of immunoreactive kinin were significantly decreased (-73.4±4.3% and -84.2± 5.3% change from control values, respectively). At the same time, the urine volume and urinary sodium excretion significantly decreased by 27.0±7.1% and 21.4±6.1%, respectively. In the rats receiving anti-ricin-IgG or anti-ricin-Fab, the renal function revealed no change. Bolus injection of 2.0 mg anti-KAL-Fab caused antidiuretic and antinatriuretic actions without change in GFR, renal blood flow or mean blood pressure. On the other hand, urinary PGE? fell by 39.4±18.4% following the injection of 2.0mg anti-KAL-Fab. Anti-ricin-Fab demonstrated no change in any of these parameters. The change in PGE? correlated significantly with that of urine volume (r=0.73 ; p< 0.001) and urinary sodium excretion (r=0.61 ; p<0.01). Thus, these findings show that 1) anti-KAL-Fab probably suppresses the renal kallikrein activity in the luminal site of the nephron ; 2) inhibition of kinin generation causes decreases in the water and sodium excretions in normotensive conscious rats ; 3) these renal changes are not dependent on renal hemodynamic alteration ; and 4) luminal kinin partially controls PGE? formation in the kid- neys. From these results, it was concluded that kinin on the luminal site of the distal nephron plays a role in the regulation of water and sodium excretion acting as a paracrine hormone, either directly or via PGE?. | |||||
書誌情報 |
札幌医学雑誌 = The Sapporo medical journal en : The Sapporo medical journal 巻 62, 号 2, p. 75-85, 発行日 1993-04-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0036-472X | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | 札幌医科大学医学部 |