{"created":"2023-05-15T09:07:25.396377+00:00","id":14783,"links":{},"metadata":{"_buckets":{"deposit":"418ee7e7-7e5d-4f40-8e45-23db0b06979a"},"_deposit":{"created_by":14,"id":"14783","owners":[14],"pid":{"revision_id":0,"type":"depid","value":"14783"},"status":"published"},"_oai":{"id":"oai:sapmed.repo.nii.ac.jp:00014783","sets":["1658:1659:1724"]},"author_link":["26165","26166","26162","26163","26164"],"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1993-04-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"85","bibliographicPageStart":"75","bibliographicVolumeNumber":"62","bibliographic_titles":[{"bibliographic_title":"札幌医学雑誌 = The Sapporo medical journal"},{"bibliographic_title":"The Sapporo medical journal","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Recent findings have suggested that the endogenous renal kallikrein-kinin system acts on the renal water-sodium metabolism at the distal nephron as a paracrine hormone system. The present study was designed to determine the acting site, and secondarily, to evaluate the role of the kallikrein-kinin system on renal hemodynamics and prostaglandin E? （PGE?） production. IgG （anti-KAL-IgG） and Fab fragment （anti-KAL-Fab） were isolated from monoclonal antibody against rat glandular kallikrein ; both anti-KAL-IgG and anti-KAL-Fab were found to inhibit glandular kallikrein activity in vitro. Next, purified IgG （anti-ricin-IgG） and Fab fragment （anti-ricin-Fab） from monoclonal antibody to ricin, which is a vegetable protein and does not exist in mammals, were tested ; neither anti-ricin-IgG nor anti-ricin-Fab blocked kallkrein activity in vitro. The anti-KAL-Fab were observed to pass through the glomeruli and reach the luminal site of the distal nephron, but anti-KAL-IgG did not pass through the glomerulus. In this study, both anti-KAL-Fab and anti- KAL-IgG were administered to unanesthetized normotensive rats. When 1.0 mg anti-KAL-Fab was administered, urinary kallikrein activity （kininogenase activity） and the excretion of immunoreactive kinin were significantly decreased （-73.4±4.3% and -84.2± 5.3% change from control values, respectively）. At the same time, the urine volume and urinary sodium excretion significantly decreased by 27.0±7.1% and 21.4±6.1%, respectively. In the rats receiving anti-ricin-IgG or anti-ricin-Fab, the renal function revealed no change. Bolus injection of 2.0 mg anti-KAL-Fab caused antidiuretic and antinatriuretic actions without change in GFR, renal blood flow or mean blood pressure. On the other hand, urinary PGE? fell by 39.4±18.4% following the injection of 2.0mg anti-KAL-Fab. Anti-ricin-Fab demonstrated no change in any of these parameters. The change in PGE? correlated significantly with that of urine volume （r=0.73 ; p< 0.001） and urinary sodium excretion （r=0.61 ; p<0.01）. Thus, these findings show that 1） anti-KAL-Fab probably suppresses the renal kallikrein activity in the luminal site of the nephron ; 2） inhibition of kinin generation causes decreases in the water and sodium excretions in normotensive conscious rats ; 3） these renal changes are not dependent on renal hemodynamic alteration ; and 4） luminal kinin partially controls PGE? formation in the kid- neys. From these results, it was concluded that kinin on the luminal site of the distal nephron plays a role in the regulation of water and sodium excretion acting as a paracrine hormone, either directly or via PGE?.","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"26165","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Saitoh, Shigeyuki"}]},{"nameIdentifiers":[{"nameIdentifier":"26166","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Shimamoto, Kazuaki"}]}]},"item_7_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15114/smj.62.75","subitem_identifier_reg_type":"JaLC"}]},"item_7_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"札幌医科大学医学部"}]},"item_7_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0036-472X","subitem_source_identifier_type":"ISSN"}]},"item_7_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"斎藤, 重幸"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"島本, 和明"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Oscar, A.  Carretero"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-07-31"}],"displaytype":"detail","filename":"n0036472X62275.pdf","filesize":[{"value":"751.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"n0036472X62275.pdf","url":"https://sapmed.repo.nii.ac.jp/record/14783/files/n0036472X62275.pdf"},"version_id":"c40a1960-1f50-4282-b190-2a8ca5a5800d"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Kallikrein-kinin system","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Water-sodium metabolism","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Prostaglandin E?","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Monoclonal antibody","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Fab-fragment","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"非麻酔下正常血圧ラットにおける腎Kallikrein-Kinin系抑制時の腎水・ナトリウム代謝","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"非麻酔下正常血圧ラットにおける腎Kallikrein-Kinin系抑制時の腎水・ナトリウム代謝","subitem_title_language":"ja"},{"subitem_title":"The Effects of the Suppression of Renal Kallikrein-Kinin System on the Water and Sodium Metabolism in Normotensive Conscious Rats","subitem_title_language":"en"}]},"item_type_id":"7","owner":"14","path":["1724"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2019-07-31"},"publish_date":"2019-07-31","publish_status":"0","recid":"14783","relation_version_is_last":true,"title":["非麻酔下正常血圧ラットにおける腎Kallikrein-Kinin系抑制時の腎水・ナトリウム代謝"],"weko_creator_id":"14","weko_shared_id":-1},"updated":"2023-12-13T02:23:46.544314+00:00"}