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Effect of Retinoic Acid on the Growth and the Expression of the Human Papillomavirus 16 E6 and E7 Genes of the Cervical Carcinoma Cell Lines
https://doi.org/10.15114/tr.28.63
https://doi.org/10.15114/tr.28.638617327f-dbf1-444b-ae02-2e4c732f3807
名前 / ファイル | ライセンス | アクション |
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||||||||||
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公開日 | 2019-07-31 | |||||||||||||||
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タイトル | Effect of Retinoic Acid on the Growth and the Expression of the Human Papillomavirus 16 E6 and E7 Genes of the Cervical Carcinoma Cell Lines | |||||||||||||||
言語 | en | |||||||||||||||
言語 | ||||||||||||||||
言語 | eng | |||||||||||||||
キーワード | ||||||||||||||||
言語 | en | |||||||||||||||
主題Scheme | Other | |||||||||||||||
主題 | Retinoic acid | |||||||||||||||
キーワード | ||||||||||||||||
言語 | en | |||||||||||||||
主題Scheme | Other | |||||||||||||||
主題 | Cervical carcinoma cell line | |||||||||||||||
キーワード | ||||||||||||||||
言語 | en | |||||||||||||||
主題Scheme | Other | |||||||||||||||
主題 | Growth suppression | |||||||||||||||
キーワード | ||||||||||||||||
言語 | en | |||||||||||||||
主題Scheme | Other | |||||||||||||||
主題 | Human papillomavirus type 16 (HPV16) | |||||||||||||||
キーワード | ||||||||||||||||
言語 | en | |||||||||||||||
主題Scheme | Other | |||||||||||||||
主題 | E6/E7 gene expression | |||||||||||||||
資源タイプ | ||||||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||
資源タイプ | departmental bulletin paper | |||||||||||||||
ID登録 | ||||||||||||||||
ID登録 | 10.15114/tr.28.63 | |||||||||||||||
ID登録タイプ | JaLC | |||||||||||||||
著者 |
Furuhata, Tomohisa
× Furuhata, Tomohisa
× Yamashita, Toshiharu
× Hirata, Koichi
× Sugawara, Kenji
× Fujinaga, Kei
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抄録 | ||||||||||||||||
内容記述タイプ | Abstract | |||||||||||||||
内容記述 | Human papillomavirus type 16 (HPV16) is involved in the development of cervical carcinoma and its viral gene products E6 and E7 are believed to be essential for the carcinogenic process. We analyzed the effect of retinoic acid (RA) on the growth and/or HPV16 E6 and E7 gene expression in the HPV16-containing cervical carcinoma cell lines SiHa, CaSki and QG-U and the HPV-free cell lines C33A and EBC-1. RA (10-6 M) suppressed the growth of SiHa cells by more than 90% and of QG-U cells by about 40% ; however, the growth of CaSki, C33A and EBC-1 cells was not affected by RA. Semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) was applied for investigation of the relationship between RA and the level of HPV16 E6/E7 mRNA. As a result, the E6/E7 mRNA level in SiHa and QG-U was reduced by RA by about 60% and 75%, respectively, while RA had no obvious effect on the E6 and E7 gene expression in CaSki cells. The chloramphenicol acetyltransfer-ase (CAT) assay showed that transcription driven by HPV16 non-coding region (NCR) was suppressed by RA by about 75%. These results suggest that at least a part of the growth suppression of SiHa and QG-U by RA is mediated by sup-pression of E6 and E7 expression. | |||||||||||||||
書誌情報 |
Tumor Research en : Tumor Research 巻 28, p. 63-76, 発行日 1993 |
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収録物識別子タイプ | ISSN | |||||||||||||||
収録物識別子 | 0041-4093 | |||||||||||||||
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出版タイプ | VoR | |||||||||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||
出版者 | ||||||||||||||||
出版者 | Sapporo Medical University |
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Cite as
Furuhata, Tomohisa, Yamashita, Toshiharu, Hirata, Koichi, Sugawara, Kenji, Fujinaga, Kei, 1993, Effect of Retinoic Acid on the Growth and the Expression of the Human Papillomavirus 16 E6 and E7 Genes of the Cervical Carcinoma Cell Lines: Sapporo Medical University, 63–76 p.
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