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p53 Point Mutations in Fine-Needle Aspirated Breast Cancer Cells and Their DNA Ploidy Patterns
https://doi.org/10.15114/tr.30.47
https://doi.org/10.15114/tr.30.474f39da8d-4620-43b5-a85c-42efc85379df
名前 / ファイル | ライセンス | アクション |
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n004140933047.pdf (452.4 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2019-07-31 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | p53 Point Mutations in Fine-Needle Aspirated Breast Cancer Cells and Their DNA Ploidy Patterns | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Breast cancer | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Aspiration biopsy | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | p53 mutation | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | DNA ploidy | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | PCR-SSCP | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
ID登録 | ||||||
ID登録 | 10.15114/tr.30.47 | |||||
ID登録タイプ | JaLC | |||||
著者 |
Sato, Takashi
× Sato, Takashi× Yuyama, Yuichi× Watabe, Kosho× Takahashi, Katsumune× Okazaki, Akira× Denno, Ryuichi× Toda, Kazunori× Okazaki, Minoru |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Breast cancer cells can be obtained derectly from the patient with minimal damage by fine-needle sampling. The method of aspiration biopsy cytology (ABC) by fine-needle aspiration was developed that enabled us to prepare cancer cell nuclei for detection of p53 gene mutation by PCR-SSCP. Fine-needle sam-pling was successfully performed on 49 patients with breast tumor. Thirty-one aspirated specimens (63.3%) produced enough malignant material for assess-ment ; 18 were diagnosed as being cytologically benign. In 49 patients, surgical specimens from the same tumors were examined for DNA aneuploid and S-phase fraction using flow cytometry. Twenty-four of the 31 breast cancer specimens (77.4%) were classified as aneuploid pattern. p53 gene mutations were detected in 15 patients (48.4%) ; all showed point mutations. In this series, no significant correlation was found with respect to the factors such as aneuploidy, p53 muta-tion, and ER in various tumor size or menopausal status. On the contrary, there was a significant relationship between aneuploidy pattern and p53 mutation ; fifteen p53 mutated cancer cell samples showed all aneuploid patterns. Further-more, these 2 factors showed a tendency of high incidence in advanced clinical stage, histologic grade, and tumor size. Thus, taking ABC into consideration, a combined examination of p53 mutation and DNA histograms derived from flow cytometry in fresh tissue samples could be a valuable tool in clinical use for biological evaluation of breast cancer. | |||||
書誌情報 |
Tumor Research en : Tumor Research 巻 30, p. 47-56, 発行日 1995 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0041-4093 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | Sapporo Medical University |