{"created":"2023-05-15T09:07:01.001874+00:00","id":14233,"links":{},"metadata":{"_buckets":{"deposit":"68289fad-f741-4f54-bfa7-6e5a10037167"},"_deposit":{"created_by":15,"id":"14233","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"14233"},"status":"published"},"_oai":{"id":"oai:sapmed.repo.nii.ac.jp:00014233","sets":["1658:1661:1710"]},"author_link":["23767","23768","23769","23770","23771","23772","23773","23774"],"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1995","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"56","bibliographicPageStart":"47","bibliographicVolumeNumber":"30","bibliographic_titles":[{"bibliographic_title":"Tumor Research"},{"bibliographic_title":"Tumor Research","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Breast cancer cells can be obtained derectly from the patient with minimal damage by fine-needle sampling. The method of aspiration biopsy cytology (ABC) by fine-needle aspiration was developed that enabled us to prepare cancer cell nuclei for detection of p53 gene mutation by PCR-SSCP. Fine-needle sam-pling was successfully performed on 49 patients with breast tumor. Thirty-one aspirated specimens (63.3%) produced enough malignant material for assess-ment ; 18 were diagnosed as being cytologically benign. In 49 patients, surgical specimens from the same tumors were examined for DNA aneuploid and S-phase fraction using flow cytometry. Twenty-four of the 31 breast cancer specimens (77.4%) were classified as aneuploid pattern. p53 gene mutations were detected in 15 patients (48.4%) ; all showed point mutations. In this series, no significant correlation was found with respect to the factors such as aneuploidy, p53 muta-tion, and ER in various tumor size or menopausal status. On the contrary, there was a significant relationship between aneuploidy pattern and p53 mutation ; fifteen p53 mutated cancer cell samples showed all aneuploid patterns. Further-more, these 2 factors showed a tendency of high incidence in advanced clinical stage, histologic grade, and tumor size. Thus, taking ABC into consideration, a combined examination of p53 mutation and DNA histograms derived from flow cytometry in fresh tissue samples could be a valuable tool in clinical use for biological evaluation of breast cancer.","subitem_description_type":"Abstract"}]},"item_7_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15114/tr.30.47","subitem_identifier_reg_type":"JaLC"}]},"item_7_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Sapporo Medical University"}]},"item_7_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0041-4093","subitem_source_identifier_type":"ISSN"}]},"item_7_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sato, Takashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yuyama, Yuichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Watabe, Kosho"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takahashi, Katsumune"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Okazaki, Akira"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Denno, Ryuichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Toda, Kazunori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Okazaki, Minoru"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-07-31"}],"displaytype":"detail","filename":"n004140933047.pdf","filesize":[{"value":"452.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"n004140933047.pdf","url":"https://sapmed.repo.nii.ac.jp/record/14233/files/n004140933047.pdf"},"version_id":"5dcf72e0-442e-4349-aea3-f7167319eb03"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Breast cancer","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Aspiration biopsy","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"p53 mutation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA ploidy","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"PCR-SSCP","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"p53 Point Mutations in Fine-Needle Aspirated Breast Cancer Cells and Their DNA Ploidy Patterns","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"p53 Point Mutations in Fine-Needle Aspirated Breast Cancer Cells and Their DNA Ploidy Patterns","subitem_title_language":"en"}]},"item_type_id":"7","owner":"15","path":["1710"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2019-07-31"},"publish_date":"2019-07-31","publish_status":"0","recid":"14233","relation_version_is_last":true,"title":["p53 Point Mutations in Fine-Needle Aspirated Breast Cancer Cells and Their DNA Ploidy Patterns"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-12-13T02:45:08.339432+00:00"}