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Immortalization of Primary Rat Cells by Reciprocal Complementations of Human Papillomavirus Type 16 E7 and Adenovirus Type 5 E1A Mutants
https://doi.org/10.15114/tr.32.1
https://doi.org/10.15114/tr.32.1353fe6e9-8732-4d19-aa88-96c5ebb8a0a3
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
n00414093321.pdf (3.3 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||||||||||||
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| 公開日 | 2019-07-31 | |||||||||||||||||
| タイトル | ||||||||||||||||||
| タイトル | Immortalization of Primary Rat Cells by Reciprocal Complementations of Human Papillomavirus Type 16 E7 and Adenovirus Type 5 E1A Mutants | |||||||||||||||||
| 言語 | en | |||||||||||||||||
| 言語 | ||||||||||||||||||
| 言語 | eng | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 言語 | en | |||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| 主題 | Adenovirus type 5 (Ad5) E1A | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 言語 | en | |||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| 主題 | Adenovirus type 5 (Ad5) E1B 19K | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 言語 | en | |||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| 主題 | Human papillomavirus type 16 (HPV16) E7 | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 言語 | en | |||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| 主題 | Immortalization | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 言語 | en | |||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| 主題 | Complementation | |||||||||||||||||
| 資源タイプ | ||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||
| 資源タイプ | departmental bulletin paper | |||||||||||||||||
| ID登録 | ||||||||||||||||||
| ID登録 | 10.15114/tr.32.1 | |||||||||||||||||
| ID登録タイプ | JaLC | |||||||||||||||||
| 著者 |
Kimura, KagiichiSachiko
× Kimura, KagiichiSachiko
× Yamashita, Toshiharu
× Yoshida, Koichi
× Ishidal, Setsuko
× Mori, Michio
× Fujinaga, Kei
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| 抄録 | ||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||
| 内容記述 | Primary baby rat kidney (BRK) cells were transfected with two different non-immortalizing mutants of adenovirus type 5 (Ad5) E1A(s) and/or human papillomavirus type 16 (HPV16) E7(s) to test whether immortalization was induced by the genetic complementation. Co-transfection of an RB-nonbinding E1A mutant (d1922/947) and N-terminal E7 mutant (2PRO) formed transformed foci on BRK cells, but a combination of an N-terminal E1A mutant (NTdl598) and RB-nonbinding E7 mutant (24GLY) did not. However, NTd1598 plus 24GLY as well as dl922/947 plus 2PRO produced immortalized BRK cell lines. The cell lines immortalized by dl922/947 plus 2PRO showed a morphology similar to those immortalized by Ad5 E1A ; while cell lines immortalized by NTdl598 plus 24GLY showed a morphology similar to those immortalized by HPV16 E7. These results suggest that the RB-binding regions of Ad5 E1A and HPV16 E7 are mutually replaceable, and that the N-terminal function of E1A is essential for focus formation and maintenance of transformation morphology. A combination of RB-nonbinding mutants, E1A dl922/947 plus E7 24GLY, was able to immortalize BRK cells when introduced together with the Ad5 E1B 19K gene. The Ad5 E1B 55K and Bcl-2, which also possess anti-apoptotic activity, did not increase colony formation of and/or immortalize BRK cells when co-transfected with dl922/947 plus 24GLY. In a CAT assay E1B 19K, but not Bc1-2, enhanced E2F-dependent CAT transactivation by Ad5 ElA or dl922/947. Thus, it is suggested that E1B 19K might complement RB-nonbinding mutants by means of two activities, suppression of apoptosis and enhancement of E2F-dependent transactivation. | |||||||||||||||||
| 書誌情報 |
Tumor Research en : Tumor Research 巻 32, p. 1-21, 発行日 1997 |
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| 収録物識別子タイプ | ISSN | |||||||||||||||||
| 収録物識別子 | 0041-4093 | |||||||||||||||||
| 著者版フラグ | ||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||
| 出版者 | ||||||||||||||||||
| 出版者 | Sapporo Medical University | |||||||||||||||||
