{"created":"2023-05-15T09:07:00.418162+00:00","id":14219,"links":{},"metadata":{"_buckets":{"deposit":"785b63cf-69fa-4fc0-83dd-8f749b179f46"},"_deposit":{"created_by":15,"id":"14219","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"14219"},"status":"published"},"_oai":{"id":"oai:sapmed.repo.nii.ac.jp:00014219","sets":["1658:1661:1712"]},"author_link":["23680","23681","23682","23683","23684","23685"],"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1997","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"21","bibliographicPageStart":"1","bibliographicVolumeNumber":"32","bibliographic_titles":[{"bibliographic_title":"Tumor Research"},{"bibliographic_title":"Tumor Research","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Primary baby rat kidney (BRK) cells were transfected with two different non-immortalizing mutants of adenovirus type 5 (Ad5) E1A(s) and/or human papillomavirus type 16 (HPV16) E7(s) to test whether immortalization was induced by the genetic complementation. Co-transfection of an RB-nonbinding E1A mutant (d1922/947) and N-terminal E7 mutant (2PRO) formed transformed foci on BRK cells, but a combination of an N-terminal E1A mutant (NTdl598) and RB-nonbinding E7 mutant (24GLY) did not. However, NTd1598 plus 24GLY as well as dl922/947 plus 2PRO produced immortalized BRK cell lines. The cell lines immortalized by dl922/947 plus 2PRO showed a morphology similar to those immortalized by Ad5 E1A ; while cell lines immortalized by NTdl598 plus 24GLY showed a morphology similar to those immortalized by HPV16 E7. These results suggest that the RB-binding regions of Ad5 E1A and HPV16 E7 are mutually replaceable, and that the N-terminal function of E1A is essential for focus formation and maintenance of transformation morphology. A combination of RB-nonbinding mutants, E1A dl922/947 plus E7 24GLY, was able to immortalize BRK cells when introduced together with the Ad5 E1B 19K gene. The Ad5 E1B 55K and Bcl-2, which also possess anti-apoptotic activity, did not increase colony formation of and/or immortalize BRK cells when co-transfected with dl922/947 plus 24GLY. In a CAT assay E1B 19K, but not Bc1-2, enhanced E2F-dependent CAT transactivation by Ad5 ElA or dl922/947. Thus, it is suggested that E1B 19K might complement RB-nonbinding mutants by means of two activities, suppression of apoptosis and enhancement of E2F-dependent transactivation.","subitem_description_type":"Abstract"}]},"item_7_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15114/tr.32.1","subitem_identifier_reg_type":"JaLC"}]},"item_7_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Sapporo Medical University"}]},"item_7_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0041-4093","subitem_source_identifier_type":"ISSN"}]},"item_7_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kimura, KagiichiSachiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamashita, Toshiharu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yoshida, Koichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ishidal, Setsuko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mori, Michio"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fujinaga, Kei"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-07-31"}],"displaytype":"detail","filename":"n00414093321.pdf","filesize":[{"value":"3.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"n00414093321.pdf","url":"https://sapmed.repo.nii.ac.jp/record/14219/files/n00414093321.pdf"},"version_id":"64c0d866-1ce9-4455-b75a-df7bf419f41e"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Adenovirus type 5 (Ad5) E1A","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Adenovirus type 5 (Ad5) E1B 19K","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Human papillomavirus type 16 (HPV16) E7","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Immortalization","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Complementation","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Immortalization of Primary Rat Cells by Reciprocal Complementations of Human Papillomavirus Type 16 E7 and Adenovirus Type 5 E1A Mutants","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Immortalization of Primary Rat Cells by Reciprocal Complementations of Human Papillomavirus Type 16 E7 and Adenovirus Type 5 E1A Mutants","subitem_title_language":"en"}]},"item_type_id":"7","owner":"15","path":["1712"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2019-07-31"},"publish_date":"2019-07-31","publish_status":"0","recid":"14219","relation_version_is_last":true,"title":["Immortalization of Primary Rat Cells by Reciprocal Complementations of Human Papillomavirus Type 16 E7 and Adenovirus Type 5 E1A Mutants"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-12-13T02:44:42.547010+00:00"}