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Dried Feces Spots as an Alternative DNA Source : Detection of K-ras Mutations in Colorectal Cancer Screening
https://doi.org/10.15114/tr.36.7
https://doi.org/10.15114/tr.36.7ee943ab3-35b8-4353-8603-81571ee70949
名前 / ファイル | ライセンス | アクション |
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n004140933617.pdf (93.1 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2019-07-31 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Dried Feces Spots as an Alternative DNA Source : Detection of K-ras Mutations in Colorectal Cancer Screening | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Stool | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Noninvasive sample | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Fecal occult blood | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Colorectal cancer | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | K-ras mutation | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Genetic screening | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
ID登録 | ||||||
ID登録 | 10.15114/tr.36.7 | |||||
ID登録タイプ | JaLC | |||||
著者 |
Yamaguchi, Akihiro
× Yamaguchi, Akihiro× Hashimoto, Naoko× Tsutae, Wataru× Seino, Kuniyoshi× Osanai, Hiroyuki× Yamamoto, Naoya× Tokino, Takashi× Sato, Noriyuki× Kikuchi, Kokichi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Detection of genetic alterations in exfoliated intestinal cells into feces is expected to be a novel approach to colorectal cancer screening. We aimed here to detect cancer-related gene mutations in routine fecal specimen; dried feces spots (DFS) on a filter paper especially designated for an automated fecal occult blood (FOB) test. DNA was simply extracted from a 3 mm disc of DFS (including around 10 mg feces) with a boiling procedure followed by a cationic surfactant extraction. K-ras common mutations, as the typical cancer-related gene alterations, were detected with a nested allele specific PCR with microfluorometry (ASP/MFL). Adequate DNA was recovered from 363/451 (80.5%) DFS specimens tested. The K-ras mutations were detected in 2/24 (8.3%) from patients with colorectal cancer and 13/315 (4.1%) from screening participants with positive FOB. No mutations were detected in 24 DFS specimens with negative FOB. The results of K-ras mutation frequency were not encouraging to achieve practically a specific dreliable method for colorectal cancer screening. However this report, identifying for the first time a cancer related gene alteration in feces using identical samples with routine FOB test, will be a step to realize a molecular genetic testing for the cancer screening. | |||||
書誌情報 |
Tumor Research en : Tumor Research 巻 36, p. 7-13, 発行日 2001 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0041-4093 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | Sapporo Medical University |