{"created":"2023-05-15T09:03:48.771867+00:00","id":8161,"links":{},"metadata":{"_buckets":{"deposit":"276dfa16-4077-4009-a5e0-48b30e2a6344"},"_deposit":{"created_by":15,"id":"8161","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"8161"},"status":"published"},"_oai":{"id":"oai:sapmed.repo.nii.ac.jp:00008161","sets":["1674:1677:1678"]},"author_link":["6768","6769","6770","6771","6772","6773","6774"],"item_2_alternative_title_18":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Restriction Fragment Length Polymorphism Analysis on Mycobacterium tuberculosis: Report No.1; Modification of hte Method"}]},"item_2_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2000","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"56","bibliographicPageStart":"52","bibliographicVolumeNumber":"27","bibliographic_titles":[{"bibliographic_title":"札幌市衛生研究所年報 = Annual Report of Sapporo City Institute of Public Health"}]}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"私たちは，結核菌のRFLP（Restriction fragment length polymorphism；制限酵素断片長多型）分析法の改良を試みた。PCRによるプローブについては245bp増幅産物をアガロースゲルから切り出してジゴキシゲニン標識した後，検出反応を行ったところ発色強度及び感度とも良好であった。RFLPについてはDNAを制限酵素消化後にフェノールを用いて制限酵素を完全に失活することにより蛍光シグナルが増強した。また，酵素標識したDNA断片の検出に，アルカリホスファターゼ活性検出用蛍光基質3-Hydroxy-N-2’-biphenyl-2-naphthalenecarboxamide phosphate ester（HNPP）を用いることにより，良好な蛍光発色が得られ，簡便にDNA断片を検出することができた。札幌市内の医療機関2施設より提供された結核患者からの分離菌株について，この方法でRFLP分析したところ，数本のバンドを有する多型性が認められ，蛍光基質HNPPの有用性が確認された。","subitem_description_type":"Abstract"}]},"item_2_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"札幌市衛生研究所"}]},"item_2_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0917-0294","subitem_source_identifier_type":"ISSN"}]},"item_2_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"川合, 常明"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"廣地, 敬"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"赤石, 尚一"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"大谷, 倫子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"藤田, 晃三"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"品川, 雅明"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"伊達, 義昭"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-07-21"}],"displaytype":"detail","filename":"n091702942752.pdf","filesize":[{"value":"104.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"n091702942752.pdf","url":"https://sapmed.repo.nii.ac.jp/record/8161/files/n091702942752.pdf"},"version_id":"0b90c368-5c02-43ac-b4ab-cdf481b7942b"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"結核菌の制限酵素多型分類 ; 第1報. 方法の検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"結核菌の制限酵素多型分類 ; 第1報. 方法の検討","subitem_title_language":"ja"}]},"item_type_id":"2","owner":"15","path":["1678"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2009-03-24"},"publish_date":"2009-03-24","publish_status":"0","recid":"8161","relation_version_is_last":true,"title":["結核菌の制限酵素多型分類 ; 第1報. 方法の検討"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-12-13T04:41:52.629230+00:00"}