@article{oai:sapmed.repo.nii.ac.jp:00015287,
 author = {正木, 章二 and 赤保内, 良和},
 issue = {3},
 journal = {札幌医学雑誌 = The Sapporo medical journal, The Sapporo medical journal},
 month = {Jun},
 note = {Various kinds of anti-free L-chain sera （A-FL） were prepared against several Bence Jones proteins （BJP） and a polyclonal L-chain followed by adequate absorption, in order to investigate the localization of hidden antigenic determinants on BJP molecules. These were divided into variable halves （VL） and constant halves （CL） by enzymic digestion. The reactions of intact BJP and these fragments to A-FL were analysed byimmunoelectrophoresis and immunodiffusion, and the following results wereobtained:1. A-FL appeared to react to a free L-chain, however it did not react to intramolecular L-chains such as whole immunoglobulins （Ig） and Ig fragments （Fab, Fc）.2. The reactions between each BJP and each A-FL showed considerable variations respectively.However, A-FL against a single BJP showed no reactions to a heterologous BJP of the same type at times. On the other hand, A-PFL which was produced by a polyclonal L-chain, however, showed a good reaction to various BJP to such an extent that A-FPL was regarded as broad spectrum antiserum. It is apparent from these results that this antiserum is highly suitable for the determination of a free L-chain.3. The enzymic digestion of BJP was carried out by using pepsin, trypsin and papain respectively. However, each BJP showed different properties in the conditions and mode of digestion, namely, type of enzyme, enzyme/BJP ratio, buffer, temperature, time etc.. The resultant products also gave rise to a considerable diversity depending on the conditions. Regarding the fragments in certain BJP both VL and CL are observed while in other BJP either VL or CL alone is observed while in still other BJP both VL and CL were not observed, in other words the results were not consistent.4. It is reasonable to presume that the antigenicity which is recognized in the form of hidden antigenic determinants by using A-FL might relate to the conformational structure of the whole molecule of a L-chain. Although it is not evident from these data whether these determinants are actually located on CL or VL, we are of the opinion that it may relate to CL from the data obtained from certain A-FL. （Received September 2, 1978 and accepted December 11, 1978）},
 pages = {225--235},
 title = {遊離Light ChainのHidden Antigenic Determinantに関する研究},
 volume = {48},
 year = {1979}
}