@article{oai:sapmed.repo.nii.ac.jp:00014980,
 author = {星, 秀樹 and 今井, 浩三},
 issue = {4},
 journal = {札幌医学雑誌 = The Sapporo medical journal, The Sapporo medical journal},
 month = {Aug},
 note = {In order to analyze the liver cell membrane antigen, anti-liver specific membrane lipoprotein （LSP） antibodies and anti-liver membrane antibodies were measured. Several monoclonal antibodies （MoAbs） were prepared against liver cell membranes. They were utilized to see if inhibition occurs by the antibodies in the serum of patients with chronic liver diseases for MoAbs to bind liver cell membranes. By use of one monoclonal antibody, we characterized the corresponding antigens on the liver cell membranes. The results obtained in this study were as follows: 1） Anti-LSP antibodies and anti-liver membrane antibodies showed higher titers in the sera of patients with autoimmune hepatitis and chronic hepatitis than in the controls. Especially the sera of patients with non-A non-B type chronic hepatitis showed significantly （p<0.01） higher titers than patients with B type chronic hepatitis. 2） In order to analyze the antigenic determinants against antibodies in the sera of patients with autoimmune hepatitis and chronic hepatitis, we immunized human liver cancer cell line PLC/PRF/5 to BALB/c mouse and prepared three monoclonal antibodies （MoAbs H1, H2, and H3）. 3） MoAb H1 was organ nonspecific and species specific, while MoAb H2 and H3 were organ non-specific as well as species non-specific. The corresponding antigen of MoAb H2 was 75K dalton in LP2 fraction of the liver homogenate described by McFarlane et al. An indirect immunoperoxidase method revealed that the determinant was well stained in the normal liver tissue, but was lost in the cancerous liver tissue. 4） Two sera （HA and KO） from patients with autoimmune hepatitis inhibited the reactivity of MoAb H1 with Chang liver cell membrane in flow cytometry. 5） Immunoblotting by use of thin layer chromatography （TLC） showed that the antigen recognized by MoAb H1 was glycolipid. 6） The method is useful for analyzing liver membrane antigens and their antibodies in chronic liver diseases.},
 pages = {423--435},
 title = {肝細胞膜抗原のモノクローナル抗体を用いた解析 : 特に慢性肝疾患患者血清中の抗体の対応抗原の検索},
 volume = {57},
 year = {1988}
}