{"created":"2023-05-15T09:07:28.660923+00:00","id":14860,"links":{},"metadata":{"_buckets":{"deposit":"2e045881-cbd4-483f-95d1-0419aa98125e"},"_deposit":{"created_by":14,"id":"14860","owners":[14],"pid":{"revision_id":0,"type":"depid","value":"14860"},"status":"published"},"_oai":{"id":"oai:sapmed.repo.nii.ac.jp:00014860","sets":["1658:1659:1721"]},"author_link":["26504","26505","26502","26503"],"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1990-06-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"286","bibliographicPageStart":"275","bibliographicVolumeNumber":"59","bibliographic_titles":[{"bibliographic_title":"札幌医学雑誌 = The Sapporo medical journal"},{"bibliographic_title":"The Sapporo medical journal","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"MoAb HA58 （IgG 1） was established by immunizing human colonic cancer BM314 cells, which were treated with IFN-γ, to BALB/c mouse. Western blotting revealed that the MoAb HA58 recognizes a 85Kd molecule which is recognized by MoAb CL207 to intercellular adhesion molecule-1 （ICAM-1）. Immunodepletion experiment showed that each antibody reacts with the different epitope on the same ICAM-1 molecule. Using this novel monoclonal antibody, the expression of the ICAM-1 antigen in the cell surface of 12 kinds of cancer cells was found to increase with IFN-γ, which was detected with flow cytometry. We established ELISA system for detecting the ICAM-1 antigen by using two MoAbs, CL207 and biotinylated HA58. We were able to detect, for the first time, a soluble form of the ICAM-1 antigen in spent medium of 11 kinds of cancer cell lines which was also increased with IFN-γ. It was also increased by TNF-α and IL-1β, although the amount was less than that of soluble ICAM-1 induced by IFN-γ. These findings suggest that the soluble form of ICAM-1 may play an important role in the immune response between tumor cells and the cells of immune system.","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"26504","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Hirata, Hiromi"}]},{"nameIdentifiers":[{"nameIdentifier":"26505","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Imai, Kohzoh"}]}]},"item_7_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15114/smj.59.275","subitem_identifier_reg_type":"JaLC"}]},"item_7_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"札幌医科大学医学部"}]},"item_7_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0036-472X","subitem_source_identifier_type":"ISSN"}]},"item_7_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"平田, 博巳"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"今井, 浩三"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-16"}],"displaytype":"detail","filename":"n0036472X593275.pdf","filesize":[{"value":"1.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"n0036472X593275.pdf","url":"https://sapmed.repo.nii.ac.jp/record/14860/files/n0036472X593275.pdf"},"version_id":"bd94f5f7-17c9-4d14-b37f-30d4c636f9f8"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ICAM-1","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Interferon-γ","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Monoclonal antibody","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Tumor cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"ELISA","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"インターフェロン-γにより発現の増強する分子ICAM-1に対する新しいモノクローナル抗体の作製と遊離型ICAM-1の検出","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"インターフェロン-γにより発現の増強する分子ICAM-1に対する新しいモノクローナル抗体の作製と遊離型ICAM-1の検出","subitem_title_language":"ja"},{"subitem_title":"Preparation of New Monoclonal Antibody to Interferon-γ Induced Molecule ICAM-1 and Detection of the Solubilized ICAM-1 Antigen with ELISA","subitem_title_language":"en"}]},"item_type_id":"7","owner":"14","path":["1721"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2019-08-16"},"publish_date":"2019-08-16","publish_status":"0","recid":"14860","relation_version_is_last":true,"title":["インターフェロン-γにより発現の増強する分子ICAM-1に対する新しいモノクローナル抗体の作製と遊離型ICAM-1の検出"],"weko_creator_id":"14","weko_shared_id":-1},"updated":"2023-12-13T02:24:27.826321+00:00"}