@article{oai:sapmed.repo.nii.ac.jp:00014860,
 author = {平田, 博巳 and 今井, 浩三},
 issue = {3},
 journal = {札幌医学雑誌 = The Sapporo medical journal, The Sapporo medical journal},
 month = {Jun},
 note = {MoAb HA58 （IgG 1） was established by immunizing human colonic cancer BM314 cells, which were treated with IFN-γ, to BALB/c mouse. Western blotting revealed that the MoAb HA58 recognizes a 85Kd molecule which is recognized by MoAb CL207 to intercellular adhesion molecule-1 （ICAM-1）. Immunodepletion experiment showed that each antibody reacts with the different epitope on the same ICAM-1 molecule. Using this novel monoclonal antibody, the expression of the ICAM-1 antigen in the cell surface of 12 kinds of cancer cells was found to increase with IFN-γ, which was detected with flow cytometry. We established ELISA system for detecting the ICAM-1 antigen by using two MoAbs, CL207 and biotinylated HA58. We were able to detect, for the first time, a soluble form of the ICAM-1 antigen in spent medium of 11 kinds of cancer cell lines which was also increased with IFN-γ. It was also increased by TNF-α and IL-1β, although the amount was less than that of soluble ICAM-1 induced by IFN-γ. These findings suggest that the soluble form of ICAM-1 may play an important role in the immune response between tumor cells and the cells of immune system.},
 pages = {275--286},
 title = {インターフェロン-γにより発現の増強する分子ICAM-1に対する新しいモノクローナル抗体の作製と遊離型ICAM-1の検出},
 volume = {59},
 year = {1990}
}