{"created":"2023-05-15T09:07:24.369994+00:00","id":14759,"links":{},"metadata":{"_buckets":{"deposit":"185204d8-e435-4833-a53c-98a9edcbeb96"},"_deposit":{"created_by":14,"id":"14759","owners":[14],"pid":{"revision_id":0,"type":"depid","value":"14759"},"status":"published"},"_oai":{"id":"oai:sapmed.repo.nii.ac.jp:00014759","sets":["1658:1659:1723"]},"author_link":["26058","26059","26060","26055","26056","26057"],"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1992-04-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"154","bibliographicPageStart":"145","bibliographicVolumeNumber":"61","bibliographic_titles":[{"bibliographic_title":"札幌医学雑誌 = The Sapporo medical journal"},{"bibliographic_title":"The Sapporo medical journal","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Molecular biological analysis was performed to clarify the epitope structure of digestive organ cancer-associated antigen MUSE11. Consequently, the following results were obtained: 1. Monoclonal antibody （MoAb） MUSE11 reacted with the synthetic peptide corresponding to the tandem repeat domain of human polymorphic epithelial mucin （PEM）. 2. A cDNA clone of 735bp was isolated from a normal human kidney cDNA library and was designated as mucin 700 by using an oligonucleotide probe corresponding to part of the tandem repeat domain. Nucleotide sequencing showed that mucin 700 consisted of 10 tandemly repeated domains. 3. Recombinant mucin 700 was produced with an expression vector, pGEX-2T, in E. Coli. MoAb MUSE11 was shown to react with this protein by Western blot analysis and sandwich enzyme immunoassay. 4. PEM core protein mRNA was expressed in both cancerous and non-cancerous regions of the stomach and the colon. It was revealed by Northern blot analysis using total RNAs extracted from surgically resected tissues. At the protein level, this was also confirmed by immunostaining with frozen tissue sections.","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"26058","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Nakagawa, Naoaki"}]},{"nameIdentifiers":[{"nameIdentifier":"26059","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Hinoda, Yuji"}]},{"nameIdentifiers":[{"nameIdentifier":"26060","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Imai, Kohzoh"}]}]},"item_7_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15114/smj.61.145","subitem_identifier_reg_type":"JaLC"}]},"item_7_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"札幌医科大学医学部"}]},"item_7_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0036-472X","subitem_source_identifier_type":"ISSN"}]},"item_7_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"仲川, 尚明"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"日野田, 裕治"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"今井, 浩三"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-07-31"}],"displaytype":"detail","filename":"n0036472X612145.pdf","filesize":[{"value":"2.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"n0036472X612145.pdf","url":"https://sapmed.repo.nii.ac.jp/record/14759/files/n0036472X612145.pdf"},"version_id":"81b49555-6037-445a-bd3f-4747c493791e"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Monoclonal antibody","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Human polymorphic epitherial mucin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Mucin core protein","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Gastric cancers","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Colonic cancers.","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"消化器癌関連MUSE11抗原の分子生物学的解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"消化器癌関連MUSE11抗原の分子生物学的解析","subitem_title_language":"ja"},{"subitem_title":"Molecular Biological Analysis of Digestive Organ Cancer-associated Antigen MUSE11","subitem_title_language":"en"}]},"item_type_id":"7","owner":"14","path":["1723"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2019-07-31"},"publish_date":"2019-07-31","publish_status":"0","recid":"14759","relation_version_is_last":true,"title":["消化器癌関連MUSE11抗原の分子生物学的解析"],"weko_creator_id":"14","weko_shared_id":-1},"updated":"2023-12-13T02:23:48.748516+00:00"}