@article{oai:sapmed.repo.nii.ac.jp:00014730,
 author = {伊林, 由美子 and 東出, 俊之 and 今井, 浩三},
 issue = {4},
 journal = {札幌医学雑誌 = The Sapporo medical journal, The Sapporo medical journal},
 month = {Aug},
 note = {In order to analyze the characteristic distribution and intensity of mRNA of alpha- fetoprotein （AFP） in liver diseases, the tissues of hepatocellular carcinoma （HCC）, liver cirrhosis （LC） and other benign liver diseases were investigated by in situ hybridization （ISH） with biotinylated AFP cDNA. These results were compared with those of AFP gene products detected by the immunohistochemical method using anti-AFP monoclonal antibody, and with those of AFP mRNA expression investigated by Northern blot （NB） analysis. In addition, the expression of AFP mRNA was studied by polymerase chain reaction （PCR） in some cases. The following results were obtained. 1. Human hepatocellular carcinoma cell line HuH-7, which produces AFP, showed positive staining using the in situ hybridization technique with a AFP cDNA probe, whereas human cell lines, which do not produce AFP, failed to show positive results. Pretreatment of HuH-7 cells with RNase aboli- shed the reactivity. Furthermore, the Northern blot technique using this cDNA probe produced a 2.1 kb band which corresponds with AFP mRNA. These results indicate that the AFP cDNA probe used in this study is specific for detecting AFP mRNA. 2. In 74% of cancerous tissues, AFP mRNA was detected by the ISH. This incidence was significantly higher than that （24%） of the NB analysis. Interestingly, the incidence of positivity for HCC with LC （HCC with LC group） was not so different from that for HCC without LC （HCC group）. Four out of 6 patients who showed less than 20ng/ml of AFP concentration in their sera showed positive results in the cancerous parts of their tissues. The incidence of positivity by the immunohistochemical staining was 44%, with localization being almost the same as that by the ISH technique. 3. In non-cancerous tissues from patients, 44% of the cases were shown to be positive by the ISH and 28% by immunohistochemical staining, whereas none were shown to be positive by the NB tech- nique. 4. Cases of liver cirrhosis, chronic hepatitis and other benign liver diseases were also analyzed. One case out of 6 LC patients and 4 out of 30 chronic hepatitis ones showed positive results using the ISH. No cases had positive results by the immunohistochemical method. 5. In the cases which showed positive results by the ISH, but were negative by the NB analysis, the PCR was carried out. The results obtained by the ISH were confirmed by this technique. 6. ISH employing liver tissue sections was useful to study the expression of AFP mRNA, and to define the preneoplastic states, such as liver cirrhosis, as well as to analyze cancerous tissues.},
 pages = {363--377},
 title = {肝癌及びその他の肝疾患組織におけるAFP mRNAの発現},
 volume = {60},
 year = {1991}
}