WEKO3
アイテム
{"_buckets": {"deposit": "2edfb1b6-5821-40cb-b50d-e48dddec859a"}, "_deposit": {"created_by": 15, "id": "15220", "owners": [15], "pid": {"revision_id": 0, "type": "depid", "value": "15220"}, "status": "published"}, "_oai": {"id": "oai:sapmed.repo.nii.ac.jp:00015220", "sets": ["1754"]}, "author_link": ["27859", "27860", "27861", "27856", "27857", "27858"], "item_7_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1981-10-01", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "5", "bibliographicPageEnd": "428", "bibliographicPageStart": "419", "bibliographicVolumeNumber": "50", "bibliographic_titles": [{"bibliographic_title": "札幌医学雑誌 = The Sapporo medical journal"}, {"bibliographic_title": "The Sapporo medical journal", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "The identification of adenoviruses by cleavage patterns with restriction endonucleases was presented. The results obtained are as follows ; 1) Adenovirus type 7 (Ad7) (strain Grider) DNA and infected cell DNA were cleaved with restriction endonuclease HindIII, BamHI, SalI and EcoRI, and fractionated by agarose gel electrophoresis. Cleavage patterns of adenovirus DNA could be clearly observed in the presence of infected cell DNAs. 2) SalI, HindIII and EcoRI cleavage patterns of Ad7 (strain Grider) DNA were identical with or very similar to those of Ad7 (strain Gomen) DNA but the differences among these DNAs were found clearly when BamHI was used for cleavage. 3) Cleavage patterns of Ad3 (strain G.B) and Ad7 (strain Grider) infected cell DNAs with HindIII, BamHI, SalI and EcoRI were studied. By cleavage with BamHI, we could distinguish between Ad3 and Ad7 DNAs. And each DNA from adenoviruses group A (Adl2 and Ad31), B (Ad3 and Ad7), C (Ad2 and Ad5), D (Ad9 and Ad13) and E (Ad4) had a specific cleavage pattern with BamHI, distinguishable from each other. These results indicate that we can identify adenovirus strains by the cleavage patterns with one or few suitable restriction endonucleases using DNA from a small amount of infected cells.", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "著者別名", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "27859", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Nobutaka, Suzuki"}]}, {"nameIdentifiers": [{"nameIdentifier": "27860", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Tetsuji, Ueno"}]}, {"nameIdentifiers": [{"nameIdentifier": "27861", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Toshiharu, Yamashita"}]}]}, "item_7_identifier_registration": {"attribute_name": "ID登録", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.15114/smj.50.419", "subitem_identifier_reg_type": "JaLC"}]}, "item_7_publisher_32": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "札幌医科大学医学部"}]}, "item_7_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0036-472X", "subitem_source_identifier_type": "ISSN"}]}, "item_7_version_type_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "鈴木, 信敬"}], "nameIdentifiers": [{"nameIdentifier": "27856", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "上野, 哲治"}], "nameIdentifiers": [{"nameIdentifier": "27857", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "山下, 利春"}], "nameIdentifiers": [{"nameIdentifier": "27858", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-23"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "n0036472X505419.pdf", "filesize": [{"value": "3.5 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 3500000.0, "url": {"label": "n0036472X505419.pdf", "url": "https://sapmed.repo.nii.ac.jp/record/15220/files/n0036472X505419.pdf"}, "version_id": "b57331e9-50b1-4340-a424-07dcbbfc497a"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "感染細胞DNAの制限酵素切断によるアデノウイルス同定 第1報 Ethidium Bromide 染色", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "感染細胞DNAの制限酵素切断によるアデノウイルス同定 第1報 Ethidium Bromide 染色", "subitem_title_language": "ja"}, {"subitem_title": "Identification of Adenoviruses by Cleavage Patterns with Restriction Endonucleases using Infected Cell DNAs. I. Ethidium Bromide Staining", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "15", "path": ["1754"], "permalink_uri": "https://doi.org/10.15114/smj.50.419", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2019-08-23"}, "publish_date": "2019-08-23", "publish_status": "0", "recid": "15220", "relation": {}, "relation_version_is_last": true, "title": ["感染細胞DNAの制限酵素切断によるアデノウイルス同定 第1報 Ethidium Bromide 染色"], "weko_shared_id": -1}
感染細胞DNAの制限酵素切断によるアデノウイルス同定 第1報 Ethidium Bromide 染色
https://doi.org/10.15114/smj.50.419
https://doi.org/10.15114/smj.50.419ee0ffe56-77ff-4f99-9a72-3f5f5ec61999
名前 / ファイル | ライセンス | アクション |
---|---|---|
n0036472X505419.pdf (3.5 MB)
|
|
Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2019-08-23 | |||||
タイトル | ||||||
言語 | ja | |||||
タイトル | 感染細胞DNAの制限酵素切断によるアデノウイルス同定 第1報 Ethidium Bromide 染色 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Identification of Adenoviruses by Cleavage Patterns with Restriction Endonucleases using Infected Cell DNAs. I. Ethidium Bromide Staining | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
ID登録 | ||||||
ID登録 | 10.15114/smj.50.419 | |||||
ID登録タイプ | JaLC | |||||
著者 |
鈴木, 信敬
× 鈴木, 信敬× 上野, 哲治× 山下, 利春 |
|||||
著者別名 | ||||||
姓名 | Nobutaka, Suzuki | |||||
著者別名 | ||||||
姓名 | Tetsuji, Ueno | |||||
著者別名 | ||||||
姓名 | Toshiharu, Yamashita | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The identification of adenoviruses by cleavage patterns with restriction endonucleases was presented. The results obtained are as follows ; 1) Adenovirus type 7 (Ad7) (strain Grider) DNA and infected cell DNA were cleaved with restriction endonuclease HindIII, BamHI, SalI and EcoRI, and fractionated by agarose gel electrophoresis. Cleavage patterns of adenovirus DNA could be clearly observed in the presence of infected cell DNAs. 2) SalI, HindIII and EcoRI cleavage patterns of Ad7 (strain Grider) DNA were identical with or very similar to those of Ad7 (strain Gomen) DNA but the differences among these DNAs were found clearly when BamHI was used for cleavage. 3) Cleavage patterns of Ad3 (strain G.B) and Ad7 (strain Grider) infected cell DNAs with HindIII, BamHI, SalI and EcoRI were studied. By cleavage with BamHI, we could distinguish between Ad3 and Ad7 DNAs. And each DNA from adenoviruses group A (Adl2 and Ad31), B (Ad3 and Ad7), C (Ad2 and Ad5), D (Ad9 and Ad13) and E (Ad4) had a specific cleavage pattern with BamHI, distinguishable from each other. These results indicate that we can identify adenovirus strains by the cleavage patterns with one or few suitable restriction endonucleases using DNA from a small amount of infected cells. | |||||
書誌情報 |
札幌医学雑誌 = The Sapporo medical journal en : The Sapporo medical journal 巻 50, 号 5, p. 419-428, 発行日 1981-10-01 |
|||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0036-472X | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | 札幌医科大学医学部 |