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ラット後根神経節細胞の突起形成期における形態変化 1. 発生初期におけるIn Vivo及びIn Vitroでの観察
https://doi.org/10.15114/smj.54.381
https://doi.org/10.15114/smj.54.381a36ebd98-57bf-401c-8831-cfb6b4c1347b
名前 / ファイル | ライセンス | アクション |
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n0036472X544381.pdf (4.4 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2019-08-23 | |||||
タイトル | ||||||
言語 | ja | |||||
タイトル | ラット後根神経節細胞の突起形成期における形態変化 1. 発生初期におけるIn Vivo及びIn Vitroでの観察 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Morphological Changes of Rat Dorsal Root Ganglion Cells in the Process-forming Period I. Observations In Vivo and In Vitro in Early Stages of Development | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Cell culture | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Cell transformation | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Dorsal root ganglion | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Rat | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Unipolarization | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
ID登録 | ||||||
ID登録 | 10.15114/smj.54.381 | |||||
ID登録タイプ | JaLC | |||||
著者 |
二宮, 孝文
× 二宮, 孝文 |
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著者別名 | ||||||
姓名 | Ninomiya, Takafumi | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Rat dorsal root ganglia in early stages of development were digested with trypsin to remove the connective tissues. Then dissociated ganglion cells in vivo and in vitro were observed with lightand scanning electron microscopes to clarify the transforming process from bipolar to pseudounipolar cells. 1. At the prenatal stage from the 13th to 16th day, about 70% of all ganglion cells were bipolar. After the 17th day of the prenatal stage, the population of pseudounipolar cells had increased to about 65% on the 17th day and to about 90% after the 18th day. However, about 5% of ganglion cells were still bipolar in the 1-day postnatal rat. This transforming process of the ganglion cells was confirmed with the scanning electron microscope by observing dissociated ganglion cells. 2. When the dissociated ganglion cells were seen in vitro, the cells yielded two or more processes at the early stage of culture and the processes made networks between the different cells. Bell-shaped bipolar cells were confirmed in culture by the scanning electron microscope, but no further transformation was observed due to fibroblast proliferation. However, when silver impregnation was used, complete unipolarized ganglion cells were observed. On the other hand, when fibroblasts were inhibited in long-term culture, no pseudounipolar cells were seen in light- and scanning electron microscopy. This probably means that the three-dimensional space, which contain fibroblasts, is needed for the transformation from bipolar cells to pseudounipolar cells. 3. According to successive observation by light- and scanning electron microscopes in vivo and in vitro, unipolarization of the dorsal root ganglion cells seemed not due to the fusion of the two processes but due to the elongation of the cell body near the approaching two processes. | |||||
書誌情報 |
札幌医学雑誌 = The Sapporo medical journal en : The Sapporo medical journal 巻 54, 号 4, p. 381-391, 発行日 1985-08-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0036-472X | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | 札幌医科大学医学部 |