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インターフェロン-γにより発現の増強する分子ICAM-1に対する新しいモノクローナル抗体の作製と遊離型ICAM-1の検出
https://doi.org/10.15114/smj.59.275
https://doi.org/10.15114/smj.59.2757406aece-a049-4a2f-bd50-6ec423d979af
名前 / ファイル | ライセンス | アクション |
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n0036472X593275.pdf (1.4 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2019-08-16 | |||||
タイトル | ||||||
言語 | ja | |||||
タイトル | インターフェロン-γにより発現の増強する分子ICAM-1に対する新しいモノクローナル抗体の作製と遊離型ICAM-1の検出 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Preparation of New Monoclonal Antibody to Interferon-γ Induced Molecule ICAM-1 and Detection of the Solubilized ICAM-1 Antigen with ELISA | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | ICAM-1 | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Interferon-γ | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Monoclonal antibody | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Tumor cells | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | ELISA | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
ID登録 | ||||||
ID登録 | 10.15114/smj.59.275 | |||||
ID登録タイプ | JaLC | |||||
著者 |
平田, 博巳
× 平田, 博巳× 今井, 浩三 |
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著者別名 | ||||||
姓名 | Hirata, Hiromi | |||||
著者別名 | ||||||
姓名 | Imai, Kohzoh | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | MoAb HA58 (IgG 1) was established by immunizing human colonic cancer BM314 cells, which were treated with IFN-γ, to BALB/c mouse. Western blotting revealed that the MoAb HA58 recognizes a 85Kd molecule which is recognized by MoAb CL207 to intercellular adhesion molecule-1 (ICAM-1). Immunodepletion experiment showed that each antibody reacts with the different epitope on the same ICAM-1 molecule. Using this novel monoclonal antibody, the expression of the ICAM-1 antigen in the cell surface of 12 kinds of cancer cells was found to increase with IFN-γ, which was detected with flow cytometry. We established ELISA system for detecting the ICAM-1 antigen by using two MoAbs, CL207 and biotinylated HA58. We were able to detect, for the first time, a soluble form of the ICAM-1 antigen in spent medium of 11 kinds of cancer cell lines which was also increased with IFN-γ. It was also increased by TNF-α and IL-1β, although the amount was less than that of soluble ICAM-1 induced by IFN-γ. These findings suggest that the soluble form of ICAM-1 may play an important role in the immune response between tumor cells and the cells of immune system. | |||||
書誌情報 |
札幌医学雑誌 = The Sapporo medical journal en : The Sapporo medical journal 巻 59, 号 3, p. 275-286, 発行日 1990-06-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0036-472X | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | 札幌医科大学医学部 |