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ln situ hybridization法によるCEA及びNCA mRNAの検出 : 大腸癌及び大腸腺腫における検索
https://doi.org/10.15114/smj.59.129
https://doi.org/10.15114/smj.59.129ca8ccff7-7421-4cff-bf75-9963dd8c9d2f
名前 / ファイル | ライセンス | アクション |
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n0036472X592129.pdf (3.7 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2019-08-16 | |||||
タイトル | ||||||
タイトル | ln situ hybridization法によるCEA及びNCA mRNAの検出 : 大腸癌及び大腸腺腫における検索 | |||||
言語 | ja | |||||
タイトル | ||||||
タイトル | Detection of CEA and NCA mRNA by In Situ Hybridization : Investigation in Colonic Cancers and Adenomas | |||||
言語 | en | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Carcinoembryonic antigen | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Nonspecific cross-reacting antigen | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | In situ hybridization | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Colonic cancer | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Colonic adenoma | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
ID登録 | ||||||
ID登録 | 10.15114/smj.59.129 | |||||
ID登録タイプ | JaLC | |||||
著者 |
伊藤, 淳
× 伊藤, 淳 |
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著者別名 | ||||||
識別子Scheme | WEKO | |||||
識別子 | 26463 | |||||
姓名 | Itoh, Jun | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | In order to study the expression of carcinoembryonic antigen (CEA) and nonspecific cross-reacting antigen (NCA) genes in colonic disorders, the expression of CEA and NCA mRNA in colonic cancers, noncancerous colonic mucosa and colonic adenomas was investigated by in situ hybridization. The results were compared with those of CEA gene products by the immunohistochemical method using anti-CEA monoclonal antibody. Furthermore, in some cases of colonic cancers, the expression of CEA and NCA mRNA was investigated by Northern blot analysis. The results are summarized as follows: 1. It was possible to detect specific mRNA for CEA and NCA from human cultured cell lines by in situ hybridization using probes from the 3'noncoding regions of CEA and NCA cDNA. 2. In 80% of colonic cancerous tissues, CEA mRNA was detected by in situ hybridization. The distribution of CEA mRNA corresponded with the immunohistochemical staining pattern of the CEA product. In noncancerous colonic mucosa, however, CEA mRNA was not detected by in situ hybridization. 3. The expression of NCA mRNA in colonic cancerous tissues detected by in situ hybridization and Northern blot analysis was stronger than that in noncancerous colonic mucosa. This suggests that NCA mRNA may be a useful tumor marker in histological studies. 4. The expression of CEA and NCA mRNA in colonic adenomas detected by in situ hybridization was stronger than that in noncancerous colonic mucosa. This suggests that colonic adenomas have a higher potential for malignancy than noncancerous colonic mucosa. Hereafter, it is important to compare the gene expression of CEA and NCA between cancer in adenoma and de novo cancer. | |||||
書誌情報 |
札幌医学雑誌 = The Sapporo medical journal en : The Sapporo medical journal 巻 59, 号 2, p. 129-141, 発行日 1990-04-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0036-472X | |||||
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出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | 札幌医科大学医学部 |