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アミロイド関連分子AM34抗原エピトープの解析と臨床応用
https://doi.org/10.15114/smj.62.175
https://doi.org/10.15114/smj.62.175035fb8a3-0cbe-43a4-a922-426d7e76f520
名前 / ファイル | ライセンス | アクション |
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n0036472X623175.pdf (3.2 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2019-07-31 | |||||
タイトル | ||||||
言語 | ja | |||||
タイトル | アミロイド関連分子AM34抗原エピトープの解析と臨床応用 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Analysis of the Epitope of an Amyloid-related Molecule, AM34 Antigen, and Its Clinical Application | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Amyloidosis | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | cDNA cloning | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Factor H | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Factor H-related molecule | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Alzheimer's disease | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
ID登録 | ||||||
ID登録 | 10.15114/smj.62.175 | |||||
ID登録タイプ | JaLC | |||||
著者 |
太田, 雅彦
× 太田, 雅彦× 伊東, 文生× 今井, 浩三 |
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著者別名 | ||||||
姓名 | Ota, Masahiko | |||||
著者別名 | ||||||
姓名 | Itoh, Fumio | |||||
著者別名 | ||||||
姓名 | Imai, Kohzoh | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Antigen corresponding to monoclonal antibody (MoAb) AM34, which had been generated from the fusion of mouse myeloma cells with splenocytes obtained from a BALB/c mouse that had been immunized with crude amyloid proteins extracted from the amyloid kidney of a patient with rheumatoid arthritis, was investigated for cDNA cloning by immunoscreening. The amino acid sequence deduced from the nucleotide sequences was then compared with the previously reported ones. It was consequently revealed that the amino acid sequence of the antigen reacted with MoAb AM34 corresponded with the Oterminal site of factor H, which is a complementary co-factor. Western blot analysis followed by immunoprecipitation with MoAb AM34 or anti-factor H poly- clonal antibody revealed that each antibody reacted with the same molecule of 150 KD, suggesting that the AM34 antigen is complement factor H or a very closely related molecule. Immunohistological analysis with MoAb AM34 revealed that the epitope is located in the amyloid deposits resulting from secondary amyloidosis, Alzheimer's disease or Down's syndrome. It is there- fore concluded that factor H or factor H-related protein exists in the above-mentioned amyloid tis- sues. | |||||
書誌情報 |
札幌医学雑誌 = The Sapporo medical journal en : The Sapporo medical journal 巻 62, 号 3, p. 175-186, 発行日 1993-06-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0036-472X | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | 札幌医科大学医学部 |