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消化器癌関連MUSE11抗原の分子生物学的解析
https://doi.org/10.15114/smj.61.145
https://doi.org/10.15114/smj.61.145450bd0c8-0ac9-4115-86f1-08ac3438b537
名前 / ファイル | ライセンス | アクション |
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n0036472X612145.pdf (2.4 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2019-07-31 | |||||
タイトル | ||||||
言語 | ja | |||||
タイトル | 消化器癌関連MUSE11抗原の分子生物学的解析 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Molecular Biological Analysis of Digestive Organ Cancer-associated Antigen MUSE11 | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Monoclonal antibody | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Human polymorphic epitherial mucin | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Mucin core protein | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Gastric cancers | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Colonic cancers. | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
ID登録 | ||||||
ID登録 | 10.15114/smj.61.145 | |||||
ID登録タイプ | JaLC | |||||
著者 |
仲川, 尚明
× 仲川, 尚明× 日野田, 裕治× 今井, 浩三 |
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著者別名 | ||||||
姓名 | Nakagawa, Naoaki | |||||
著者別名 | ||||||
姓名 | Hinoda, Yuji | |||||
著者別名 | ||||||
姓名 | Imai, Kohzoh | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Molecular biological analysis was performed to clarify the epitope structure of digestive organ cancer-associated antigen MUSE11. Consequently, the following results were obtained: 1. Monoclonal antibody (MoAb) MUSE11 reacted with the synthetic peptide corresponding to the tandem repeat domain of human polymorphic epithelial mucin (PEM). 2. A cDNA clone of 735bp was isolated from a normal human kidney cDNA library and was designated as mucin 700 by using an oligonucleotide probe corresponding to part of the tandem repeat domain. Nucleotide sequencing showed that mucin 700 consisted of 10 tandemly repeated domains. 3. Recombinant mucin 700 was produced with an expression vector, pGEX-2T, in E. Coli. MoAb MUSE11 was shown to react with this protein by Western blot analysis and sandwich enzyme immunoassay. 4. PEM core protein mRNA was expressed in both cancerous and non-cancerous regions of the stomach and the colon. It was revealed by Northern blot analysis using total RNAs extracted from surgically resected tissues. At the protein level, this was also confirmed by immunostaining with frozen tissue sections. | |||||
書誌情報 |
札幌医学雑誌 = The Sapporo medical journal en : The Sapporo medical journal 巻 61, 号 2, p. 145-154, 発行日 1992-04-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0036-472X | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | 札幌医科大学医学部 |