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モノクローナル抗体A3D4により検出される新しい胃癌関連ムチン抗原の解析
https://doi.org/10.15114/smj.65.499
https://doi.org/10.15114/smj.65.4999ff2a4c9-d8f6-4f90-93c5-c87aee583333
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
n0036472X656499.pdf (4.7 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||
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| 公開日 | 2019-07-31 | |||||||
| タイトル | ||||||||
| タイトル | モノクローナル抗体A3D4により検出される新しい胃癌関連ムチン抗原の解析 | |||||||
| 言語 | ja | |||||||
| タイトル | ||||||||
| タイトル | A Novel Gastric Cancer-associated Mucin Antigen Defined by a Monoclonal Antibody A3D4 | |||||||
| 言語 | en | |||||||
| 言語 | ||||||||
| 言語 | jpn | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | Monoclonal antibody | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | Gastric mucin | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | Tumor-associated antigen | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | Intestinal metaplasia | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | Gastric cancer | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | departmental bulletin paper | |||||||
| ID登録 | ||||||||
| ID登録 | 10.15114/smj.65.499 | |||||||
| ID登録タイプ | JaLC | |||||||
| 著者 |
明石, 浩史
× 明石, 浩史
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| 著者別名 | ||||||||
| 識別子Scheme | WEKO | |||||||
| 識別子 | 25782 | |||||||
| 姓名 | Akashi, Hirofumi | |||||||
| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | In this study, to attempt to develop useful markers for gastric cancers, we have purified and deglycosylated gastric mucin and established monoclonal antibodies （MoAbs）. Their characterization was immunohistochemically performed and the following results were obtained: 1） Since a MoAb designated A3D4 among established MoAbs was shown to react with gastric cancer with high frequency, but not with normal gastric epithelium, this MoAb was analysed in this study. 2） Among normal digestive organs, only the colon and gall bladder were positive for MoAb A3D4. 3） The incidence of positivity in gastric cancer was 92% for well differentiated adenocarcinoma （n=13）, 60% for moderately differentiated adenocarcinoma （n=15） and 33% for poorly differentiated adenocarcinoma （n=12）. 4） Intestinal metaplasia （IM） with chronic gastritis or gastric ulcer was negative for MoAb A3D4, whereas 8 out of 13 cases （62%） of IM with gastric cancer ere positive. 5） Western blot analysis using the lysate from normal colon tissues revealed a highmolecular-weight （>300 kD）, smear-like band. 6） With immunohistochemical analysis, the reactivity of MoAb A3D4 was increased when fixed cultured cells were pretreated with periodic acid, O-glycanase or N-glycosidase, while it was slightly decreased by pretreatment with trypsin or protease V8. 7） There was no reactivity with the synthetic peptide encompassing the tandem-repeat sequence of MUC2 or MUC3. These data suggest that MoAb A3D4 detects a novel gastric cancer-associated mucin antigen whose epitope may be peptide in nature. | |||||||
| 書誌情報 |
札幌医学雑誌 = The Sapporo medical journal en : The Sapporo medical journal 巻 65, 号 6, p. 499-509, 発行日 1996-12-01 |
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| ISSN | ||||||||
| 収録物識別子タイプ | ISSN | |||||||
| 収録物識別子 | 0036-472X | |||||||
| 著者版フラグ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
| 出版者 | ||||||||
| 出版者 | 札幌医科大学医学部 | |||||||
