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Partial Catalytic Domains of New Protein-tyrosine Kinases Cloned from cDNA Amplified by Polymerase Chain Reaction
https://doi.org/10.15114/tr.28.77
https://doi.org/10.15114/tr.28.778d039abb-e71a-4dba-b9ad-c265f7307c53
名前 / ファイル | ライセンス | アクション |
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n004140932877.pdf (1.0 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2019-07-31 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Partial Catalytic Domains of New Protein-tyrosine Kinases Cloned from cDNA Amplified by Polymerase Chain Reaction | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Protein-tyrosine kinase | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Polymerase chain reaction | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Catalytic domain | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | PCR library | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
ID登録 | ||||||
ID登録 | 10.15114/tr.28.77 | |||||
ID登録タイプ | JaLC | |||||
著者 |
Sasaki, Hiroko
× Sasaki, Hiroko× Nagura, Kazuko× Kotani, Kiyoshi× Sasaki, Terukatsu |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | A feature common to all members of the protein-tyrosine kinase (PTK) family is a highly conserved catalytic domain which is characteristic of this group. Degenerate oligonucleotide primers corresponding to two of the most highly conserved regions of the PTK catalytic domain were designed to amplify cDNA sequences of restricted subfamilies of PTKs from rat brain mRNA in the polymerase chain reaction (PCR). A third degenerate oligonucleotide primer corresponding to a highly conserved, PTK subfamily-specific sequence located between the two sequences mentioned above was also used to amplify cDNA sequences of PTKs of novel subfamilies from rat brain mRNA. pBluescript PCR libraries were constructed from the PCR-amplified cDNA. The PCR libraries were then screened by DNA sequencing for PTK-related sequences. Several sequences were identified that, on the basis of sequence comparison with known PTKs in GenBank, may encode new PTKs. | |||||
書誌情報 |
Tumor Research en : Tumor Research 巻 28, p. 77-86, 発行日 1993 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0041-4093 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | Sapporo Medical University |