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Establishment of Monitoring System to Detect Single Copy DNA Included in One Genome but not in Another Using Representational Difference Analysis
https://doi.org/10.15114/tr.31.21
https://doi.org/10.15114/tr.31.21a81297f7-c24e-4ccc-b27a-a8c03850cb80
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
n004140933121.pdf (1.4 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||||||||||||
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| 公開日 | 2019-07-31 | |||||||||||||||||
| タイトル | ||||||||||||||||||
| タイトル | Establishment of Monitoring System to Detect Single Copy DNA Included in One Genome but not in Another Using Representational Difference Analysis | |||||||||||||||||
| 言語 | en | |||||||||||||||||
| 言語 | ||||||||||||||||||
| 言語 | eng | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 言語 | en | |||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| 主題 | Gene subtraction | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 言語 | en | |||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| 主題 | Adapter | |||||||||||||||||
| 資源タイプ | ||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||
| 資源タイプ | departmental bulletin paper | |||||||||||||||||
| ID登録 | ||||||||||||||||||
| ID登録 | 10.15114/tr.31.21 | |||||||||||||||||
| ID登録タイプ | JaLC | |||||||||||||||||
| 著者 |
Mita, Hiroaki
× Mita, Hiroaki
× Itoh, Fumio
× Kakiuchi, Hideki
× Toyota, Minoru
× Hinoda, Yuji
× Imai, Kohzoh
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| 抄録 | ||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||
| 内容記述 | Polyrnerase chain reaction (PCR) -coupled subtractive procedure, representa-tional difference analysis (RDA) , is an efficient method to find the differences between two complex genomes. RDA has been applied to detect genetic lesions in cancer, the identification of unknown pathogens from the genomes, and the isolation of polymorphic markers. However, characterization of various clones obtained by RDA is time consuming and laborious work, and it is of great impor-tance to monitor whether RDA really works. To establish a monitoring system to detect single copy target DNA, we studied whether RDA could detect four fragments of non-human DNA which were added in one genome but not in another. We were able to successfully detect the target DNAs which were mixed at the ratio of single and ten copies per haploid genome using RDA with some modification of the original protocol. We confirmed that RDA was sensi-tive and effective enough to detect such genetic lesions as occurred in cancer cells. The target DNA used in this model could be utilized as a positive control in other applications of RDA. | |||||||||||||||||
| 書誌情報 |
Tumor Research en : Tumor Research 巻 31, p. 21-30, 発行日 1996 |
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| 収録物識別子タイプ | ISSN | |||||||||||||||||
| 収録物識別子 | 0041-4093 | |||||||||||||||||
| 著者版フラグ | ||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||
| 出版者 | ||||||||||||||||||
| 出版者 | Sapporo Medical University | |||||||||||||||||
