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Structural and Expressional Alterations of Episomal and Integrated Human Papillomavirus Type 16 in Precancerous Lesions and Carcinomas of the Cervix.
https://doi.org/10.15114/tr.31.73
https://doi.org/10.15114/tr.31.73c2ce39f5-4f52-4c38-aaff-d8e6305c72c0
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
n004140933173.pdf (1.8 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||||||||||||||||
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| 公開日 | 2019-07-31 | |||||||||||||||||||||
| タイトル | ||||||||||||||||||||||
| タイトル | Structural and Expressional Alterations of Episomal and Integrated Human Papillomavirus Type 16 in Precancerous Lesions and Carcinomas of the Cervix. | |||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||
| 言語 | ||||||||||||||||||||||
| 言語 | eng | |||||||||||||||||||||
| キーワード | ||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||
| 主題 | Human papillomavirus 16 (HPV 16) | |||||||||||||||||||||
| キーワード | ||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||
| 主題 | Polymerase chain reaction | |||||||||||||||||||||
| キーワード | ||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||
| 主題 | Physical state | |||||||||||||||||||||
| キーワード | ||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||
| 主題 | Genome deletion | |||||||||||||||||||||
| キーワード | ||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||
| 主題 | mRNA | |||||||||||||||||||||
| キーワード | ||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||
| 主題 | Reverse transcription | |||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||
| 資源タイプ | departmental bulletin paper | |||||||||||||||||||||
| ID登録 | ||||||||||||||||||||||
| ID登録 | 10.15114/tr.31.73 | |||||||||||||||||||||
| ID登録タイプ | JaLC | |||||||||||||||||||||
| 著者 |
Nishikawa, Akira
× Nishikawa, Akira
× Yamashita, Toshiharu
× Shimada, Masamitsu
× Fujinaga, Yukako
× Yamakawa, Yasushi
× Fukushima, Michio
× Kudo, Ryuichi
× Fujinaga, Kei
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| 抄録 | ||||||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||||||
| 内容記述 | HPV infection has long been implicated in the development of cervical car-cinoma. We have analyzed the HPV 16 genome structure and expression of the viral mRNA in cervical intraepithelial neoplasias (CINs) and cervical car-cinomas by using modified polymerase chain reaction (PCR) methods. Genome structure has been determined by PCR using multi-primer sets which are located in each open reading frames and then these results have been compared with the physical state of the viral DNA determined by two-dimensional electrophoresis. Furthermore, we have analyzed the expression of HPV 16 mRNA and genome structure using DNA and RNA simultaneously extracted from CINs and cervical carcinomas using PCR and reverse transcription (RT-) PCR. Our data showed that the DNA regions from the El to Ll region were delet-ed in two of three CINs containing episomal HPV 16 and three out of seven cervical carcinomas containing integrated HPV 16. However, the E6/E7 region was conserved in all the HPV 16-positive samples. RT-PCR analysis has determined the presence of mRNA species which could encode the E6, E6*I, E6*II, E7, E2, E2 ? C, E1^E4, E1^E2 ? C, E4, E2 ? C-E5 and L2 proteins. The overall results of DNA and mRNA analyses in cervical lesions indicated that the expression patterns of the early and late transcripts studied were not specifically related to the grade of malignancy and the physical state or the deletion of the viral genome. Furthermore, alterations in the splicing pat-terns of HPV 16 transcripts may not be involved in tumor progression. | |||||||||||||||||||||
| 書誌情報 |
Tumor Research en : Tumor Research 巻 31, p. 73-88, 発行日 1996 |
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| ISSN | ||||||||||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||||||||||
| 収録物識別子 | 0041-4093 | |||||||||||||||||||||
| 著者版フラグ | ||||||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||||
| 出版者 | ||||||||||||||||||||||
| 出版者 | Sapporo Medical University | |||||||||||||||||||||
