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Identification of SH3-domain Binding Proteins from Cell and Tissue Extracts by Using Peptide-Mass Fingerprinting
https://doi.org/10.15114/tr.35.57
https://doi.org/10.15114/tr.35.577220c57f-2ecb-4871-90e2-e9f7edd268b2
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
n0041409335157.pdf (516.2 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||||||||||||
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| 公開日 | 2019-07-31 | |||||||||||||||||
| タイトル | ||||||||||||||||||
| タイトル | Identification of SH3-domain Binding Proteins from Cell and Tissue Extracts by Using Peptide-Mass Fingerprinting | |||||||||||||||||
| 言語 | en | |||||||||||||||||
| 言語 | ||||||||||||||||||
| 言語 | eng | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 言語 | en | |||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| 主題 | Signal transduction | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 言語 | en | |||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| 主題 | SH3 domain | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 言語 | en | |||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| 主題 | MALDI-TOF-MS | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 言語 | en | |||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| 主題 | Peptide-mass fingerprinting | |||||||||||||||||
| 資源タイプ | ||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||
| 資源タイプ | departmental bulletin paper | |||||||||||||||||
| ID登録 | ||||||||||||||||||
| ID登録 | 10.15114/tr.35.57 | |||||||||||||||||
| ID登録タイプ | JaLC | |||||||||||||||||
| 著者 |
Ishino, Masaho
× Ishino, Masaho
× Aoto, Hiroshi
× Sasaki, Hiroko
× Matsumura, Fumio
× Yamashiro, Shigeko
× Sasaki, Terukatsu
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| 抄録 | ||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||
| 内容記述 | Efs is a Fyn-binding protein with an SH3 domain and multiple tyrosine phosphorylation sites,and it functions as a possible modulator in intracellular signal transduction.To better understand signaling through Efs,we searched for Efs-binding proteins by using immobillized SH3 domains fused to glutathione S-transferase (GST).Proteins which bound to the affinity matrices from cell and tissue extracts were analyzed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS).By this method,it was possible to identify multiple at one time.However,special care was necessary to eliminate nonspecific blinding of cytoskeletal proteins,including actin,myosin and tropomyosin;the amounts of these proteins present in the cell lysate were large and signaficant amounts were found to bind to the affinity matrices. The preclearing process did not work well to eliminate these cyroskeletal fractions and a specific elution procedure was necessary to selectively identify signaling proteins. | |||||||||||||||||
| 書誌情報 |
Tumor Research en : Tumor Research 巻 35, p. 57-62, 発行日 2000 |
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| 収録物識別子タイプ | ISSN | |||||||||||||||||
| 収録物識別子 | 0041-4093 | |||||||||||||||||
| 著者版フラグ | ||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||
| 出版者 | ||||||||||||||||||
| 出版者 | Sapporo Medical University | |||||||||||||||||
